Ultrasonic intensification of sorption and desorption processes during the isolation of virginiamycin from the cultural broth of Streptomyces sp. S 15-30

D. A. Durnikin, E. S. Yacenko, I. Yu. Evdokimov, V. B. Akopyan, V. V. Dhavakhiya, V. A. Savushkin, V. I. Glagolev, A. I. Ovchinnikov


Supplementation of fermentation media with synthetic resins is used in the process of biotechnological production of antibiotics and other biologically active substances to prevent the auto-inhibition of the biosynthesis processes due to the binding of secreted target metabolites to a sorbent and their removal from the fermentation volume. The efficiency of a sorbent application may be improved via the ultrasonic stimulation of the antibiotic sorption from cultural broth during fermentation, and the similar stimulation of the reverse process (desorption) during its isolation and purification. In this study, the possibility of the use of ultrasound to improve the processes of sorption and desorption of virginiamycin, a feed antibiotic produced by the highly active strain Streptomyces sp. S 15-30, to/from an Amberlite XAD-16 synthetic resin selectively binding this antibiotic, has been evaluated. According to the obtained results, the ultrasonic treatment with frequency of 22 kHz and acoustic energy density up to 0.05 W·s/cm3 does not disrupt cell membranes, i.e., does not violate biotechnological processes. At the same time, such treatment increases the sorption capacity of the sorbent in ~1.4 times. The similar ultrasonic treatment of a sorbent at the acoustic field with energy density of 0.5 W·s/cm3 almost tenfold accelerates desorption of virginiamycin from the resin but does not destroy the sorbent and provides a possibility to its re-use after regeneration. The revealed effects of the ultrasonic treatment may be integrated into the technology of the virginiamycin biosynthesis, isolation, and purification to improve the efficiency of its industrial production.


virginiamycin; sorption/desorption; intensification; ultrasound

Full Text:



Akopyan, V.B., Bambura, M.V., Rukhman, A.A., Nongayar, B. (2009). Device for measurement of the ultrasonic radiation power [Ustroistvo dlya izmereniya moshchnosti ultrazvukovogo izlucheniya]. Patent RU 2421694 (in Russian).

Akopyan, V.B., Ershov, Yu.A. (2016). Fundamentals of the ultrasound interaction with biological obkects [Osnovy vzaimodeistviya ultrazvuka s biologicheskimi ob’ektami]. Moscow: YURAIT (in Russian).

Dzhavakhiya, V., Savushkin, V., Ovchinnikov, A., Glagolev, V., Savelyeva, V., Popova, E., Novak, N., Glagoleva, E. (2016). Scaling up a virginiamycin production by a high-yield Streptomyces virginiae VKM Ac-2738D strain using adsorbing resin addition and fed-batch fermentation under controlled conditions. 3Biotech, 6, Article ID 240.

Phillips, T., Chase, M., Wagner, S., Renzi, C., Powell, M., DeAngelo, J.,

Michels, P. (2013). Use of in situ solid-phase adsorption in microbial natural product fermentation development. Journal of Industrial Microbiology and Biotechnology, 40, 411–425.

Savushkin, V.A., Dzhavakhiya, V.V., Glagoleva, E.V., Savel'eva, V.V., Popova, E.D., Ovchinnikov, A.I., Glagolev, V.I., Novak, N.V., Durnikin, D.A. (2016). Development of highly active virginiamycin-producing strain and improvement of its productivity using synthetic adsorbing resins. Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University, 6 (3), 195–208 (in Russian).

Sirotyuk, M.G. (2008). Acoustic cavitation [Akusticheskaya kavitatsiya]. Moscow: Nauka (in Russian).

Smirnova, V.V., Nazarenko, O.B. (2012). Effect of preparation conditions and ultrasonic treatment on the sorption activity of titanium dioxide. Proceedings of IX Intern. Conf. of Students and Young Scientists “Prospects of the development of fundamental sciences”, Tomsk, Russia, 484-486 (in Russian).

Srivastava, P. Goyal, S., Tayade, R. (2014) Ultrasound-assisted adsorption of reactive blue 21 dye on TiO¬2 in the presence of some rare earths (La, Ce, Pr&Gd). Canadian Journal of Chemical Engineering, 92(1), 41-51.

Sushkova, V.I., Berezina O.V., Yarotskiy, S.V. (2012). Approaches to the intensification of the butyric acid biosynthesis. Khimija Rastitel’nogo Syr’ja, 1, 171-180 (in Russian).

Verbanov, V.S., Pogadaev, V.I., Verbanov, A.V. (1992). Method for intensification of molecular sorption from water solutions [Sposob intensifikatsii molekulyarnoi sorbtsii iz vodnyh rastvorov]. Patent SU 1775167 (in Russian).

DOI: http://dx.doi.org/10.15421/2017_71

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

© 2017 Ukrainian Journal of Ecology. ISSN 2520-2138