Application of lithium ascorbate for regulation of lipid-cholesterol metabolism and glutathione redox system in pregnant sows

K.S. Ostrenko, V.P. Galochkina, V.A. Galochkin

Abstract


The study is aimed at developing a method for using a new, organic drug – lithium ascorbate. The aim of the work is to develop a new, more effective and more physiological way to combat all forms of stress in farm animals to increase productivity. Lithium ascorbate is an adaptogen preparation capable of becoming one of the new effective and physiological elements of pork production biotechnology rationalization. The experiment was carried out on 5 groups of pregnant sows of Landrace breed (4 experimental and 1 control sows) with 5 heads each. After 30 days of fruitful insemination of sows 1, 2, 3 and 4 groups received daily with food lithium ascorbate in powder form in the dose of 10; 5; 2; 0.5 mg/kg of live weight, respectively. Weighing was carried out before the introduction of the drug. Repeated weighing was carried out in 2 and 3 months after fertilization and just before farrowing. In the blood plasma was determined dialdehyde, restored glutathione, oxidized glutathione, triacylglycerol, total cholesterol, total protein, lipoprotein fraction is HDL, LDL, VLDL, thiol-disulfide ratio (SH/SS); activity of superoxide dismutase and glutathione peroxidase. It is established that the introduction of feed sows at a dosage of 10, 5 and 2 mg/kg of body weight, ascorbate, lithium exhibits a pronounced adaptogen and stress-protection properties, supported on a physiological level, the dynamics of cortisol and progesterone during pregnancy. Lithium ascorbate contributes to increase of nonspecific resistance, growth of gestating sows, the tread in relation to technological and spontaneous stressors. The obtained data indicate that lithium ascorbate in sows in all studied parameters was effective, affecting lipid-cholesterol metabolism, glutathione reduction system and as a result, increasing the safety, live weight and reproductive function of sows. The experimental verification of the developed dose, scheme and method of administration, lithium ascorbate based on a set of biochemical and physiological parameters confirmed the validity of the proposed concept on the possibility of creating with its help new methods of effective and physiological management of behavioral reactions, non-specific resistance and productivity of animals.


Keywords


gestating sows;lithium ascorbate;antioxidant status;reduction of glutathione;lipid-cholesterol metabolism;stress;productivity

Full Text:

PDF

References


Babaylova, G.P., Perevoyko, J.A. (2008). Vlijanie promyshlennoj tehnologii na nekotorye pokazateli krovi svinomatok. Voprosy fiziologii, soderzhanija, kormoproizvodstva i kormlenija, selekcii sel'skohozjajstvennyh zhivotnyh, biologii pushnyh zverej i ptic. Proceed. II Int. Conf. Kirov (in Russian).

Bazaian, F.S., Grigorian, G.F. (2006). Molecular and chemical basis of emotional states and reinforcements. Advances in Physiological Sciences, 37(1), 68-83 (in Russian).

Charmandari, E., Tsigos, C., Chrousos, G. (2005). Endocrinology of the stress response. Annual Review of Physiology, 67, 259-28.

Degroote, J., Joris, M., Claeys, E., Ovyn, A., De Smet, S. (2013). Changes in the pig small intestinal mucosal glutathione kinetics after weaning. Journal of Animal Science. 90. 359-361. DOI: 10.2527/jas.53809.

Galochkin V.A., Galochkina V.P., Ostrenko K.S. (2009). Development of theoretical foundations and creation of anti-stress drugs of new generation. Agricultural Biology, 2, 43-55 (in Russian).

Galochkin, V.A., Boryaev, G.I., Agafonova, A.V., Galochkina, V.P. (2016). The use of nootropic adaptogen new generation for the regulation of the intensity and direction of metabolic processes in the body, pregnant sows and suckling piglets. Problems of Productive Animal Biology, 1, 5-29 (in Russian).

Galochkin, V.A., Cherepanov, G.G. (2013). Nonspecific resistance in food-producing animals: difficulties of identification, problems and solutions. Problems of Productive Animal Biology, 1, 5-29 (in Russian).

Ginter, E., Bobek, P., Jurcovicova, M. (2016). Role of L-Ascorbic Acid in Lipid Metabolism (pp. 381–393). In Ascorbic Acid: Chemistry, Metabolism, and Uses. Chapter 19. Advances in Chemistry, 200. DOI: 10.1021/ba-1982-0200.ch019

Kolesnichenko, L.S., Bardymova, T.P., Verlan, N.V., Sergeev E.S., Sergeev M.P. (2009). Antioxidant system of glutathione in patients with diabetes. Siberian Medical Journal, 1, 31-33 (in Russian).

Lavoie, J.C., Mohamed, I., Nuyt, A.M., Elremaly, W., Rouleau, T. (2018). Impact of SMOFLipid on Pulmonary Alveolar Development in Newborn Guinea Pigs. JPEN J Parenter Enteral Nutr. DOI: 10.1002/jpen.1153

Likidlilid, A.N., Patchanans, S., Poldee, Peerapatdit, T. (2007). Glutathione and glutathione peroxidase in patients with diabetes type 1 diabetes. J. med. Association. Thai, 90(9), 1759-1767.

Lu, S.C. (2009). Regulation of glutathione synthesis. Molecular Aspects of Medicine, 30(1-2), 42–59. DOI: http://doi.org/10.1016/j.mam.2008.05.005

Morozov. S.V., Dolgikh, V.T., Poluektov, V.L. (2005). The Activation of lipid peroxidation is a pathogenic factor of acute dysfunction in pancreatitis. Bulletin Siberian Branch of Russian Academy of Medical Sciences, 4, 30-35 (in Russian).

Nazarenko, G.I., Kishkun, A.A. (2002). Clinic evaluation of laboratory results. Moscow. Medicine (in Russian).

Nciri, R., Allagui, M., Vincent, C., Murat, J.C., Croute, F., El Feki, A. (2009). The effects of subchronic lithium administration in male Wistar mice on some biochemical parameters. Hum Exp Toxicol, 8(10), 641-646. DOI: 10.1177/0960327109106486.

Oruch, R., Elderbi, M.A., Khattab, H.A., Pryme, I.F., Lund, A. (2014). Lithium: a review of pharmacology, clinical uses, and toxicity. Eur J Pharmacol, 5, 464-473.

Ovsepian, L.M., Kazarian, G.V., Zakharian, A.V., Zakarian, G.V., L'vov, M.V. (2010). Effect of cysteine lithium salt on lipid peroxidation in the model of dopaminergic system disorder. Eksp Klin Farmakol, 73(7), 16-8 (n Russian).

Pronin, A.V., Gromova, O.A., Sardaryan, I.S., Torshin, I.Y., Stel'mashuk, E.V., Ostrenko, K.S., Aleksandrova, O.P., Genrikhs, E.E., Khaspekov, L.G. (2016). Adaptogenic and neuroprotective effects of lithium ascorbate. Zh Nevrol Psikhiatr Im S S Korsakova, 116(12), 86-91. DOI: 10.17116/jnevro201611612186-91 (in Russian).

Romanenko V.N., Boyko, I.A. (2015). Effect of synthetic thimogen on blood protein indicators under stimulation of exchange processes of swine. Proceedings of Orenburg State Agrarian University, 3(53), 194-198 (in Russian).

Roux, M., Dosseto, A. (2017). From direct to indirect lithium targets: a comprehensive review of omics data. Metallomics. 9(10), 1326-1351. DOI: 10.1039/c7mt00203c.

Semenov, V.S., Rachkov, I.G., Kononova, L.V., Cherepanova, N.F. (2012). About pig breeding in Russia and the region. Collection of Scientific Papers of Stavropol Research Institute of Animal Husbandry and Feed Production, 1(5), 29-32 (in Russian).

Yogeeta, S.K., Hanumantra, R.B., Gnanapragasam, A., Senthilkumar, S., Subhashini, R., Devaki, T. (2006). Attenuation of abnormalities in the lipid metabolism during experimental myocardial infarction induced by isoproterenol in rats: beneficial effect of ferulic acid and ascorbic acid. Basic Clin Pharmacol Toxicol, 98(5), 467-72. DOI: 10.1111/j.1742-7843.2006.pto_335.x




DOI: http://dx.doi.org/10.15421/2018_310

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.




Since April 2018 Journal changed the editorial policy and starts to be published exclusively in English, and changed its main site into www.ujecology.com

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

© 2017 Ukrainian Journal of Ecology. ISSN 2520-2138