The nitrate metabolism enzyme indicator role in the environment state changes conditions

O.M. Vasilyuk, A.Y. Pakhomov


The paper reflects analyzes of Cd impact on the total activity (nM pyruvic acid/ml s) of aspartate aminotransferase (AST, EC nitrogen metabolism in Glechoma hederacea L. leaves subject (as model) which dominated in the research area (in natural floodplain oak with Stellaria holostea L.) in conditions of Cd pollution (as anthropogenic press) and digging activity by Mammalia (as biotic action, with Talpa europaea L., European mole, as model),) and their combine action. The Cd was introduced in the form of salts Cd(NO3)2 in the concentrations: 0.25, 1.25 and 2,5 g/m2, equivalent to the inclusion of Cd in 1,5 and 10 doses of MAC on experimental sites. When adding Cd, the content of doses (5 mg/kg soil MAC of Cd) was taken into account. It was founded the increasing of the AST activity on 26% (with adding the Cd salts at a dose of 1 МAС and digging activity by Talpa europaea L) according to control (1 MAC Cd), witch it proved the non-specific reaction on stress. With Cd concentration 5 and 10 MAC we observed the repression of the enzymes activity according to controls (5 and 10 MAC Cd) on 10% and 50% in accordance. The protective properties by T. europaea L. hadn’t positive results. The transferase enzyme activity according to another control (the area, is without pollution of Cd and digging activity by T. europaea L.) reflected the increasing AST enzyme activity from 166% tо 218% (in presence 1 and 5 MAC Cd) and reduction around 46% (in presence 10 MAC Cd). The digging activity by T. europaea L. lowered the toxic metal effect and the normalisation of the nitrogen metabolism by increasing the activity of AST from 55% to 266%, from 318% to 291% (AST, 1 та 5 MAC Cd). The digging activity by Mammalia did not contribute the metal toxic effect under the Cd 10 MAC. Thus, using the different representatives of zoocoenosis promotes improvement in the Steppe Dnieper at low concentrations of the factor has been revealed.


heavy metals;mammals;maximum allowable concentration;anthropogenic stress;aspartate aminotransferase;enzymes

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Becerril, F.R., Juárez-Vázquez, L.V., Hernández-Cervantes, S.C., Acevedo-Sandoval, O.A., Vela-Correa, G., Cruz-Chávez, E., Moreno-Espíndola, I.P., Esquivel-Herrera, A., de León-González, F. (2013). Impacts of manganese mining activity on the environment: Interactions among soil, plants and arbuscular Mycorrhiza. Environ. Contam. Toxicol. 64(2), 219–227.

Bіlanich, M.M. (2008). The current state of research of influence of heavy metals on plant life [Suchasnij stan doslіdzhennja vplivu vazhkih metalіv na roslinnij svіt]. Vіsn. Prikarpatsky Univ. Bіol. 12, 161–176 (in Ukrainian).

Boshoff,,М., Jonge, М.De., Scheifler, R., Bervoets, L. (2014). Predicting As, Cd, Cu, Pb and Zn levels in grasses (Agrostis sp. and Poa sp.) and stinging nettle (Urtica dioica) applying soil–plant transfer models. Sci. Total. Environ. 493(15), 862–871.

Dospekhov, B. A. (1985). Methods of experience of the field. Moscow, AgropromPress. (in Russian).

Douchiche, O., Driouich, A., Morvan, C. (2010). Spatial regulation of cell-wall structure in response to heavy metal stress: Cadmium-induced alteration of the methyl-etherification pattern of homogalacturonans. Ann. Bot. (Lond.) 105, 481–491

Dzyubak, O.I., Vasilyuk, O.M. (2009). Vplyv hlorydnogo zasolennja na morfometrychni ta biohimichni pokaznyky roslyn u dynamici rostu ta rozvytku [Effect of chloride salinity on morphometric and biochemical indices in the dynamics of plant growth and development]. 'Materialy I Mizhnarodnoi' Naukovoi' Konferencii'. Veber, Donets'k, 2, 231–232 (in Ukraine).

Ghavri, S.V., Singh, R.P. (2010). Phytotranslocation of Fe by biodiesel plant Jatropha curcas L. grown on iron rich waste-land soil. Braz. J. Plant Physiol. 22(4), 235–243

Hameed, A., Mahmooduzzafar, T.N.Q, Siddiqi, T.O., Iqbal, M. (2011). Differential activation of the enzymatic antioxidant system of Abelmoschus esculentus L. under CdCl2 and HgCl2 exposure. Braz. J. Plant Physiol. 23(1), 46–54.

Hu, P.J., Qiu, R.L., Senthilkumar P. (2009). Tolerance, accumulation and distribution of Zinc and Cadmium in hyperaccumulator Potentilla griffithii. Environ. Exp. Bot, 66, 317–325.

Kopittke, P.M, Blamey, F.P.C., Asher, C.J., Menzies, N.W. (2010). Trace metal phytotoxicity in solution culture: a review.J Exp Bot, 61, 945–954.

Lefcort, Н., Wehner, E.A., Cocco, P.L. (2013). Look inside get access pre-exposure to heavy metal pollution and the odor of predation decrease the ability of snails to avoid stressors. Arch. Environ. Contam. Toxicol, 64(2), 273–280.

Martí, E., Sierra, J., Cáliz, J., Montserrat, G., Vila, X.,

Garou, M.A., Cruañas, R. (2013). Ecotoxicity of Cr, Cd, and Pb on two mediterranean soils. Arch. Environ. Contam.Toxicol, 64(3), 377–387.

Martín, J.A.R., Carbonell, G., Nanos, N., Gutiérrez, S.C. (2013). Identification of soil mercury in the Spanish Islands. Envi-ron. Contam. Toxicol, 64(1), 171–179.

Mohammadian, M.A., Mobrami, Z., Sajedi, R.H. (2011). Bioactive compounds and antioxidant capacities in the flavedo tissue of two citrus cultivars under low temperature. Braz. J. Plant Physiol, 23(3), 203-208.

Naji, K.M., Devaraj, V. R. (2011). Antioxidant and other biochemical defense responses of Macrotyloma uniflorum (Lam.) Verdc. (Horse gram) induced by high temperature and salt stress. Braz. J. Plant Physiol, 23(3), 187-195.

Tsvetkova, N.M.., Pakhomov, A.Ye., Serdyuk, S.M., Yakyba, M.S. (2016). Bіological Diversity of Ukraine. The Dnipropetrovsk region. Soils. Metalls in the soils. Dnipropetrovsk: Dnipro National University Press.

Polevoy, V., V., Maximov, G.B. (1978). Methods of biochemical analysis of plants. Leningrad, Leningrad Univ. Press.

Straczek, A., Sarret, G., Manceau, A., Hinsinger, P., Geoffroy, N., Jaillard, B. (2008). Zinc distribution and speciation in roots of various genotypes of tobacco exposed to Zn.Environ. Exp. Bot, 63, 80–90.

Vasilyuk O.M. (2013a). Effect of Nickel on Aspartate Aminotransferase activity in Glechoma hederacea L. leaves subject to excretory function of Mammalia. . Zprávy vědecké ideje–2013. Proceed. IX mezinárodní vědecko - praktická conference. Praha: Education and Science.

Vasilyuk O.M. (2013b). Effect of Nickel on Alanine Aminotransferase activity in Glechoma hederacea L. leaves subject to excretory function of Mammalia. Ptoceed. Conf. Perspektywiczne opracowania sa Nauka I technikami 2013. Przemysl: Nauka I studia.

Vasilyuk, O.M., (2013c). Effect of lead on Alanine Aminotransferase activity in Glechoma hederacea L. leaves subject to digging function of Mammalia. Proceed. Conf. «Vědecky Prumysl Evropskeho kontinentu ¬2013». Praha.

Vasilyuk O.M., Dzyubak O.I. (2009). Physiological and biochemical parameters of plants as markers of a condition of environment. Proceed. I Int. Conf. Veber, Donets'k.

Vasilyuk, O.M., Pakhomov, O.Y. (2012). Effect of nickel ions on the functional activity of enzymes in the leaves of Glechoma hederatia L. under conditions of Mammalia digging activity. Proceed. VIII Conf. “Achievement of High school – 2012”. Bjalgrad, Sofija, Bolgarija (in Ukrainian).


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