Experimental study of immunoprophylactic anti-pneumococcal medicine and its immunogenic properties

Ya. V. Kysera, Yu. G. Storchak, B. V. Gutyj

Abstract


In today's veterinary science and practice, the issue of creation and introduction into the production of diagnostic and preventive immunobiological remedies remains relevant. The prevention of factor bacterial diseases is one of the main tasks of veterinary medicine. Taking into account that sufficient immunobiological reactivity and resistance of an animal`s organism is provided by the optimal composition of vitamins, macro- and microelements, and insufficient vitamin, mineral, including micromineral nutrition causes in them a violation of the function of the immune system, reduces the resistance of the organism to infections, increases the incidence, emerged the issue of developing a new preventive and immunostimulant. It was established the specific composition of streptococci. Complex researches were carried out, which included the isolation of the most virulent pneumococci from the experimental material, taken in the animal farms of Lviv region in order to develop a preventive remedy "Pneumo-Pro" against pneumococcal infection. In 29% of cases there was S. pneumoniae, 21% – S. pyogenes, 17% – S. bovis, 12% – S. agalactiae and 21% – other streptococci streptococci streptococci strains that did not respond to streptococcal serums and were not identified as S. pneumoniae. Investigation of the sensitivity of pneumococci to antibiotics has shown that isolates have been sensitive to enrofloxacin, ofloxacin. Resistance to vancomycin, cefalexin, streptomycin, amoxicillin, ampicillin, cefazolin, lincomycin and erythromycin has been established. A non-harmful and immunogenic prophylactic remedy against pathogens of pneumococcal infection was tested with laboratory animals. During the development of a prophylactic remedy, the determination of its harmlessness was investigated immunogenic properties with different ratios of components of the preventive agent "Pneumo-Pro". White mice were used in the studies. The created preventive remedy "Pneumo-Pro" in its composition contains two components: Streptococcus pneumoniae and alcoholic extract of propolis, which possesses anti-inflammatory and immunomodulatory properties. Experimental studies have shown that propolis, due to its anti-inflammatory and antioxidant properties, prevents of pneumonia. Clinical observations showed the harmlessness of the experimental samples of the remedy: no swelling and abscesses at the injection site, no increase in local temperature and body temperature, a deterioration in the general condition of laboratory animals. The expediency of the use of immunoprophylaxis, made from local isolates of an infectious agent in order to increase the resistance of animals.


Keywords


pneumococcal infection; Streptococcus pneumoniae; immunoprophylaxis; adjuvant; propolis

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DOI: http://dx.doi.org/10.15421/2018_216

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