Activation of antioxidant enzymes and content of hydrogen peroxide in winter wheat leaves by deficit of soil nitrogen

T. P. Mamenko

Abstract


We proved that the insufficient supply of soil nitrogen, induces defense reactions in winter wheat, as evidenced by the increase
in hydrogen peroxide and the activity of antioxidant enzymes in leaves. Treatment of plants with carbamide on a low nitrogen
background, induced a decrease in the content of hydrogen peroxide in leaves of varieties of winter wheat, which may indicate
a low level of oxidative processes in plants due to the inclusion of antioxidant enzymes in stress conditions. At the same time,
the intensification of the activity of antioxidant enzymes in leaves of varieties of winter wheat for the low nitrogen nutrition of
plants was recorded. Foliar dressing of winter wheat urea is regarded as a kind of stress on the plant, on the one hand, and on
the other, as a factor that stimulates of inclusion the protective mechanisms, in the above privacy and activation of work in
enzymes of ascorbate-glutathione cycle and superoxide dismutase. This contributes to a better realization of the genetic
potential of winter wheat varieties.


Keywords


Triticum aestivum L.; superoxide dismutase; glutathione reductase; ascorbate-peroxidase; hydrogen peroxide; nitrogen

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References


Baranenko, V.V. (2006). SOD in the cells of plants. Cytology, 48(6), 465–474. (In Ukrainian)

Bradford, M.A (1976). Rapid and sensitive method for the quantitation of the microgram quantities of protein utilising: the principle of protein–dye binding. Anal. Biochem, 72, 248–254.

Desikan, R., Mackerness, S.A.–H., Hancock, J.T., Neill, S.J. (2001). Regulation of the Arabidopsis Transcriptome by Oxidative Stress. Plant Physiol, 127, 159–72.

German, M. (2012). Effect of fertilizer and seed treatment preplant the formation of the physical properties of dough and baking quality of wheat soft winter. Bulletin of Poltava State Agrarian Academy, 1, 99–102. (In Ukrainian)

Giannopolitis, C.N., Ries, S.K. (1977). Superoxide Dismutase. 1. Occurrence in higher plants. Plant Physiol, 59(2), 309–314.

Halliwell, B., Foyer, C.H. (1978). Properties and physiological function of a glutathione reductase purified from Spinach leaves by affinity chromatography. Planta, 139, 388–396.

Hruskov, M., Svec, I. (2009). Wheat Hardness in Relation to Other Quality Factors. Czech J. Food Sci, 27(4), 240–248.

Kolupaev, J.E., Karpets, U.V., Oboznyi, A.I. (2011). Antioxidant system of plants, participate in cell signaling system and for adaptation to action stressors. Bulletin of Kharkiv National Agrarian University, 1(22), 6–34 (in Ukrainian).

Konturska, A.A., Palladina, T.A. (2012). Activity enzyme ascorbate–glutathione cycle in the leaves of maize seedlings under conditions of salinity and processing adaptogenic agents. Ukr. Biochem. J, 84(6), 139–144. (In Ukrainian)

Kreslavskyy, V.D., Los, D.A., Allakhverdiev, S.I., Kuznetsov, V.I. (2012). Syhnalnaya role of reactive oxygen at syresse in plants. Physiology of plants, 59(2), 163–178. (In Russian)

Li, M., Yang, X.W., Tian, X.H., Wang, S.X., Chen, Y.L. (2014). Effect of nitrogen fertilizer and foliar zinc application at different growth stages on zinc translocation and utilization efficiency in winter wheat. Cereal Res. Com, 42(1), 81–90.

Morgun, V.V., Kiriziy, D.A. (2012). Prospects and modern strategies of improving the physiological characteristics of wheat to improve performance. Physiology and biochemistry cult. Plants, 44(6), 463–483. (In Ukrainian)

Morgun, V.V., Schwartau, V.V., Kyryzyy, D.A. (2010). Physiological principles of formation peak productivity grain cereal. Physiology and biochemistry cultural plants, 42(5), 371–393. (In Ukrainian)

Mynybaeva, F.V., Gordon, L.K. (2003). Products extracellular superoxide and peroxidase activity in plants tissues under the stress. Physiology of plants, 50(3), 459– 464. (In Russian)

Nakano, Y., Asada, K. (1981). Hydrogen peroxidase is scavenged by ascorbate–specific peroxidase in spinach chloroplasts. Plant Cell Physiol, 22(5), 867–880.

Sagisaka, S. (1976). The occurrence of peroxide in a perennial plant, Populus gelrica. Plant Physiol, 57(2), 308–309.

Sedlar, O., Balik, J., Cerny, J., Peklova, L., Kulhanek, M. (2015). Nitrogen uptake by winter wheat (Triticum aestivum) depending on fertilizer application. Cereal Res. Com, 43(3), 515–524.

Sokolovska–Serhiyenko, A.G., Kiriziy, D.A. (2013). The intensity of photosynthesis and antioxidant enzymes leaves of winter wheat under different conditions of mineral nutrition physiology and biochemistry. Physiology and biochemistry cultural plants, 45(3), 206–214. (In Ukrainian)




DOI: http://dx.doi.org/10.15421/2018_199

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