Antibacterial effect of "Ecocide C" disinfectant against mycobacteria

A. P. Paliy


We presented the results of experimental studies on the bactericidal properties of the disinfectant "Ecocide С" (produced by "KRKA", Slovenia) with respect to various types of mycobacteria. The objects of research were 15 cultures of various species of mycobacteria from the collection of pathogens of infectious diseases of animals of the National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine" (Kharkov). The disinfectant "Ecocide C" had bactericidal properties with respect to various species of mycobacteria: M. avium, M. bovis, M. kansasii, M. gordonae, M. scrofulaceum, M. intracellulare, M. terrae, M. triviale, M. xenopi, M. diernhoferi, M. flavescens, M. fortuitum, M. phlei, M. smegmatis, M. thamnopheos. When exposed to bactericidal concentrations, the disinfectant "Ecocide C" caused destruction of the cell wall and cytoplasmic membrane of mycobacteria, destruction of the granular component of the cytoplasm with the formation of osmiophilic fine granular inclusions, and electron-transparent vacuoles. When we applied the disinfectant "Ecocide C" on mycobacterial cells it caused the irreversible changes in the structural elements of mycobacteria and finally the death of microorganisms. Disinfectant "Ecocide C" shown bactericidal properties against atypical mycobacteria and pathogens of tuberculosis of farm animals and poultry at a concentration of 5.0 % with exposure to the action of 24 hours and can be applied in a common complex of antituberculous activities.


mycobacterium; disinfectant; "Ecocide C"; concentration; exposure; electron microscopy

Full Text:



Amanfu, W. (2006). The situation of tuberculosis and tuberculosis control in animals of economic interest. Tuberculosis, 86(3-4), 330-335. doi: 10.1016/

Barker, R.M. (1964). The bactericidal action of low molecular weight compounds on Mycobacterium tuberculosis. J. Appl. Bacteriol., 27, 213-220. doi: 10.1111/j.1365-2672.1964.tb04905.x

Bello, T., Rivera-Olivero, I.A., de Waard, J.H. (2006). Inactivation of mycobacteria by disinfectants with a tuberculocidal label. Enferm Infecc Microbiol Clin., 24(5), 319–321. PMID: 16762258

Dermicheva, S.G., Dosanov, K.Sh., Koronelli, T.V. (1993). Incorporation of labeled precursors of macromolecular compounds into cells of pathogenic and saprophyte mycobacteria in the presence of «Dezoksone-1». Journal Microbiol. epidemiol. and immunobiol., 6, 19-20. (In Russian)

Evstaf’eva, V.O. (2009). Testing disinfectants for ascarid pigs invasion. Journal of Poltava State Agrarian Academy, 1, 101-103. (In Ukrainian)

Falkinham, J.O. (2002). Nontuberculous mycobacteria in the environment. Clin. Chest Med., 23(3), 529-551. doi:10.1016/S0272-5231(02)00014-X

Fevre, E.M., Bronsvoort, B., Hamilton, K.A., Cleaveland, S. (2006). Animal movements and the spread of infection diseases. Trends in Microbiology, 14(3), 125-131. doi:

Iakubchak, O.M. (2010). Veterinary disinfection: instruction and guidelines. Kyiv: "Company Bioprom". (In Ukrainian)

Johnson, M.M., Odell, J.A. (2014). Nontuberculous mycobacterial pulmonary infections. J Thorac Dis., 6(3), 210-220. doi: 10.3978/j.issn.2072-1439.2013.12.24

Katoch, V.M. (2004). Infections due to nontuberculous mycobacteria (NTM). Indian. J. Med. Res., 120, 290-304. ISSN 0971-5916

Kolosov, A.A., Kachkin, M.V., Tupota, S.G., Dudkin, A.L., Agapova, M.F. (2006). Perfection of the epizootological monitoring system for tuberculosis of cattle using information technology. Siberian Bulletin of Agriculture Science, 4, 52-57. (In Russian)

Kolos, Yu., Stec, V., Titarenko, V., Zelinskiy, M., Iakubchak, O., Homenko, V. (2006). On the issue of TB diagnosis in animals. Veterinary Medicine in Ukraine, 11, 10-12. (In Ukrainian)

Kotlyar, A.V. (2014). The study of pathogenic and sensitizing the properties of atypical mycobacteria isolated from cattle. Veterinary Medicine, 99, 76-78. (In Ukrainian)

Kovalenko, V.L., Nedosekov, V.V. (2011). The concept of integrated development and use of disinfectants for veterinary medicine: Monograph. Kyiv: NUBiP Ukraine. (In Ukrainian)

Naymanov, A.X., Ustinova, G.I., Tolstenko, N.G., Vangeli, E.P., Kucheruk, O.D. (2015). Non-tuberculous (atypical) mycobacteria and their sensitizing significance. Journal Veterinaria i kormlenie, 1, 19-21. (In Russian)

O’Brien, D.P., Currie, B.J., Krause, V.L. (2000). Nontuberculous mycobacterial disease in northern Australia: a case series and review of the literature. Clin. Infect. Dis., 31, 958-967. doi: 10.1086/318136

Ovdienko, N.P., Solodova, I.V. (2004). Methodical bases of an estimation of an epizootic situation on a tuberculosis of animals. Veterinary pathology, 1-2(9), 43-44. (In Russian)

Paliy, A.P., Zavgorodniy, A.I., Stegniy, B.T., Gerilovych, A.P. (2015). A study of the efficiency of modern domestic disinfectants in the system of TB control activities. Agricultural Science and Practice, 2(2), 26-31. doi: 10.15407/agrisp.2.02.026

Paliy, A.P., Stegniy, B.T., Vedmid, O.V., Zagrebelniy, V.O., Paliy, A.P. (2016). Disinfectants tuberculosis animals. Veterinary Medicine, 102, 172-176. (In Ukrainian)

Parhomenko, N.A., Piotrovich, V.A., Tindik, V.S., Romanenko, O.A., Salganska, O.O., Vigovska, L.M. (2008). Learning efficiency disinfectant "Ecocide C" in the laboratory. Veterinary Biotechnology Bulletin, 13, 98-102. (In Ukrainian)

Pavlova, I.B., Kulikovsky, A.V. (1978). Submicroscopic study of bacteria and spores under the action of peracetic acid and some aspects in the mechanism of action of the preparation. Journal Microbiology, Epidemiology and Immunobiology, 1, 37-40. (In Russian)

Polakov, A.A. (1975). Veterinary disinfection. Мoscow: Коlos. (In Russian)

Prokop’eva, N.I., Bilgaeva, A.A., Oboeva, N.A., Fedotov, I.R. (2007). Etiology of tuberculin reactions in cattle. Siberian Bulletin of Agriculture Science, 11, 51-55. (In Russian)

Rutala, W.A. (1990). APIC guideline for selection and use of disinfectants. Am. J. Infect. Control, 18(2), 99-117. PMID: 2110790

Selaninov, Yu.O., Tatarchuk, O.P., Birukova, A.V. (2009). Virulicide activity of “Ecocide C” against the causative agent of African swine fever. Veterinary Medicine of the Kuban, 5, 18-19. (In Russian)

Shkromada, O.I., Sklar, O.I. (2016). Veterinary and sanitary assessment of products of slaughter pigs by the use of disinfectants "Ecocide C" and "Virosan". Bull. of Sumy National Agrarian University, 11(39), 66-69. (In Ukrainian)

Stegniy, B.T., Gerilovich, A.P., Limanska, O.Yu., Golovko, V.O., Bilenko, V.A., Bolotin, V.I., Gerilovich, I.O., Stegniy, M.Yu., Sapko, S.A., Solodankin, O.S., Stegniy, A.B., Golovko, M.A., Vovk, S.I., Rudova, N.G. (2010). Polymerase chain reaction in the practice of veterinary medicine and biological research. Kharkiv: NТMТ. (In Ukrainian)

Sulis, G., Roggi, A., Matteelli, A., Raviglione, M.C. (2014). Tuberculosis: Epidemiology and Control. Mediterr J Hematol Infect Dis., 6(1):e2014070. doi: 10.4084/MJHID.2014.070.

Troshin, E.I., Bochkareva, L.A. (2012). Efficiency of aerosols of peroxide compounds in the disinfection of pigs' premises. Sci. notes Kazan. state. acad. vet. medicine, 212, 176-179. (In Russian)

Uikli B. (1975). Electron microscopy for beginners. Мoscow: Мir Publishers. (In Russian)

Urbanchich, A. (2008). Ecocide – safety and effectiveness. Veterinary Medicine of the Kuban, 2, 19-20. (In Russian)

Wagner, D., Young, L.S. (2004). Nontuberculous mycobacterial infections: a clinical review. Infection, 32(5), 257-270. doi: 10.1007/s15010-004-4001-4

White, D.G., McDermott, P.F. (2001). Biocides, drug resistance and microbial evolution. Curr. Opin. Microbiol., 4(3), 313-317. doi: 10.1016/S1369-5274(00)00209-5

Zavgorodniy, A.I., Kalashnik, N.V., Kochmarskiy, V.A., Busol, V.O., Paliy, A.P., Tihonov, P.M., Gorjeev, V.M. (2007). Guidelines "Determination of the bactericidal properties of disinfectants, disinfection and quality control tuberculosis farm animals". Kharkiv: NSC IEKVM. (In Ukrainian)


Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


  • There are currently no refbacks.

Since April 2018 Journal changed the editorial policy and starts to be published exclusively in English, and changed its main site into


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

© 2017 Ukrainian Journal of Ecology. ISSN 2520-2138