The seed’s ultrastructure of genus Iris L. s.l. (Iridaceae) species from Ukrainian Carpathians

O. A. Futorna, S. L. Zhygalova, V. A. Badanina


We have studied the ultrastructure of five species of genus Iris L., which grow in the Ukrainian Carpathians – I. aphylla L., I.graminea L., I. pseudacorus L., I. sibirica L. and I. sintenisii Janka. We have found that in shape seeds are rounded-fusiform (I. aphylla), D-shaped (I. pseudacorus, I. sibirica, I.graminea), pyriform (I. sintenisii). The hilum is rounded or drop-shaped, small, by position – basal (I. graminea), with well-seen roll (I. graminea, I. aphylla). The seeds of I. sibirica and I. pseudacorus have wing, that surround all the seed (wing is smaller in seeds of I. pseudacorus). The wings are absent in seeds of other species. Тhe curvature of anticlinal cell walls (straight) is common for all studied species. The seeds of all examined species characterized by polygonal cells of testa, they are isodiametric (I. aphylla, I. sintenisii, I. graminea), or prolonged (I. sibirica, I. pseudacorus) – mostly on the wing. The boundaries of cells of testa are practically not visible, anticlinal cell walls are tightly merged only in I. sintenisii seeds, in other species seeds they are seen. The anticlinal cell walls in I. aphylla and I. graminea are raised in relation to surface of periclinal cell walls. The outer periclinal cell walls are concave (I. sintenisii, I. aphylla), flat (I. graminea), or convex (I. pseudacorus, I. sibirica). The sculpture of outer periclinal cell walls is smooth (I. graminea, I. sintenisii, I. aphylla), or furrowed (I. sibirica, I. pseudacorus). Considering all the above features, we concluded that seeds of studied species are characterized by coliculate (I. sibirica, I. pseudacorus), reticulate (I. aphylla), indistinct-coliculate with slightly convex periclinal cell walls (I. graminea) or indistinct-cellular (I. sintenisii) relief.


Iris s.l.; seed ultrastructure; seeds testa; Ukrainian Carpathians; SEM

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Barthlott, W. (1981). Epidermal and seed surface characters of plants: systematic applicability and some evolutionary aspects. Nord. J. Bot. Copenhagen, 1(3), 345-355. doi: 10.1111/j.1756-1051.1981.tb00704.

Barthlott, W., Ziegler, B. (1980). Über ausziehbare helicale Zellwandverdickungen als Haft-Apparat der Samenschalen von Chiloschista lunifera (Orchidaceae). Ber. Deutsch. Bot. Ges., 93, 391–403. doi: 10.1111/j.1438-8677.1980.tb03349.

Canne, J M. (1979). A light and scanning electron microscope study of seed morphology in Agalinis (Scrophulariaceae) and its taxonomic significance. Syst. Bot., 4, 281-296.

Canne, J. M. (1980). Seed surface features in Aureolaria, Brachystigma, Tomanthera, and certain South American Agalinis (Scrophulariaceae). Syst. Bot., 5, 241-252.

Chance, G. D., Bacon, J. D. (1984). Systematic implications of seed coat morphology in Nama (Hydrophyllaceae). American Journal of Botany, 7(6), 829-842.

Chopyk, V.I., Fedoronchuk, M.M. (2015). Flora Ukrai'ns'kyh Karpat. TzOV «Terno-graf», Ternopil. (In Ukrainian).

Chuang, T. I., Heckard, L. R. (1972). Seed-coat morphology in Cordylanthus (Scrophulariaceae) and its taxonomic significance. Amer. J. Bot., 59, 258-265.

Clark, C., Jernstedt, J.A. (1978). Systematic studies of Eschscholzia (Papaveraceae). II. Seed coat microsculpturing. Syst. Bot., 3, 386-402.

Didukh, Ya.P. (ed.) (2009). Red book of Ukraine. The plant world. Globalconsulting, Kyiv. (In Ukrainian)

Dobrochaeva, D.N., Kotov, M.I., Prokudin, Ju.N. i dr. (1987). Opredelitel' vysshih rastenij Ukrainy. 1 izd. Nauk. dumka, Kiev. (In Russian).

Fayed, A.A., El Naggar, S.M. (1988). Taxonomic studies on Cruciferae in Egypt. 2. Taxonomic significance of the seed coat sculpturing in species of tribe Brassiceae. Taeckholmia, 11, 87- 95.

Fayed, A.A., El Naggar, S.M. (1996). Taxonomic studies on Cruciferae in Egypt. 4. Seed morphology and taxonomy of the Egyptian species of Lepidieae. Bull. Fac. Sci. Assiut Univ., 25, 43-50.

Fomin, O.V., Bordzilovs'kyj, Je.I. (1950). Rid Pivnyky – Iris (Tourn.) L. In Flora URSR, 3, 283-303. Academy of Sciences of Ukraine Ress, Kyiv. (In Ukrainian)

Haridasan, V.K., Mukherjee, P.K. (1993). Seed surface features in Indian Lobeliaceae. Phytomorphology, 43, 287–294.

Heyn, C.C., Herrnstadt, I. (1977). Seed coat structure of Old World Lupinus species. Bot. Not., 130, 427–435.

Heywood, V.H. (1971). Scanning electron microscopy. Systematic and evolutionary applications. London, New York, Academic Press.

Hill, R.J. (1976). Taxonomic and phylogenetic significance of seed microsculpturing in Mentzelia (Loasaceae) in Wyoming and adjacent Western states. Brittonia, 28, 86–112.

Juan, R., Pastor, J., Fernandez, I. (2000). SEM and light microscope observations on fruit and seeds in Scrophulariaceae from southwest Spain and their systematic significance. Annals of Botany, 86, 323–338. doi:10.1006/anbo.2000.1188.

Karam, M.F. (1997). Scanning electron microscope studies of seed characters in Trifolium L. (Fabaceae). Phytomorphology, 47, 51–56.

Koul, K.K., Ranjna, N., Raina, S.N. (2000). Seed coat microsculpturing in Brassica and allied genera (subtribes Brassicinae, Raphaninae, Moricandiinae). Annals of Botany, 86, 385–397. doi:10.1006/anbo.2000.1197.

Matthews, J.F., Levins, P.A. (1986). The systematic significance of seed morphology in Portulaca (Portulacaceae) under scanning electron microscopy. Syst. Bot., 11, 302–308.

Musselman, L.J., Mann, W.F. (1976). A survey of surface characteristics of seeds of Scrophulariaceae and Orobanchaceae using scanning electron microscopy. Phytomorphology, 26, 370 –378.

Newell, C.A., Hymowitz, T. (1978). Seed coat variation in Glycine Wild. subgenus Glycine (Leguminosae) by SEM. Brittonia, 30, 76–88.

Seavey, S.R., Magill, R.E., Raven, P.H. (1977). Evolution of seed size, shape and surface architecture in the tribe Epilobieae (Onagraceae). Ann. Missouri Bot. Gard., 64, 18–47.

Segarra, J.G., Mateu, I. (2001). Seed morphology of Linaria species from eastern Spain: identification of species and taxonomic implications. Bot. J. Linn. Soc., 135, 375–389.

Stern, W.T. (1992). Botanical Latin. IV Edition. David Charles, London.

Vít Bojˇnanský, Agáta Fargašová. (2007). Atlas of Seeds and Fruits of Central and East-European Flora. The Carpathian Mountains Region. Springer.

Volutsa, O.D. (2011). Iris brandzae Prodán (Iridaceae) u flori Chernivec'koi' oblasti. Aktual'ni problemy botaniky ta ekologii'. Proceed. Int. Conf. Berezne, Ukraine (In Ukrainian).

Whiffin, T., Tomb, A.S. (1972). The systematic significance of seed morphology in the neotropical capsular-fruited Melastomataceae. Amer. J. Bot., 59, 411–422.

Wofford, B.E. (1981). External seed morphology of Arenaria (Caryophyllaceae) of the southeastern United States. Syst. Bot., 6, 126–135.

Zhygalova, S.L., Futorna, O.A. (2016). Ul'trastruktura poverhni nasinyn vydiv pidrodu Xyridion (Tausch) Spach rodu Iris L. (Iridaceae) flory Ukrai'ny. Modern Phytomorphology, 9, 97–105. (In Ukrainian).


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