Pathogenicity of Simplicillium lanosoniveum to Coccus hesperidum

M. Skaptsov, S. Smirnov, M. Kutsev, O. Uvarova, T. Sinitsyna, A. Shmakov, A. Matsyura

Abstract


Simplicillium lanosoniveum isolate SSBG2 was isolated from the diseased C. hesper collected from Schefflera octophylla in greenhouse of the South-Siberian Botanical Garden, and identified based on morphological observation and ITS region analysis. The infected plants were inoculated with conidial suspension of the isolate SSBG2 in concentrations 1.0*105/mL. It was showed that after inoculation the C. hesper female adults were more vulnerable to infection. Larvae are affected to a lesser extent. The mycelium grows under the scale and cause the death of the insect. It was indicated that S. lanosoniveum had high infectivity against C. hesper. Infection symptoms appeared on day 7 after the inoculation, the infection reached the peak on day 20. Our study provides a new isolate that affects the Coccus hesperidum.


Keywords


Coccus; entomopathogenic; fungi; PCR; Simplicillium

Full Text:

PDF

References


Baiswar, P.S., Ngachan, V., Rymbai, H., Chandra, S. (2014.) Simplicillium lanosoniveum, a hyperparasite on Aecidium elaeagni-latifoliae in India. Austral. Plant Dis., Note, 9, 144. doi: 10.1007/s13314-014-0144-z

Chen, R., Huang, C., Li, J, Tsay, J. (2017). Evaluation of characteristics of Simplicillium lanosoniveum on pathogenicity to aphids and in vitro antifungal potency against plant pathogenic fungi. Int. J. Environ. Agric. Res., 3(1), 2454-1850.

Chen, R., Huang, C., Li, J., Tsay, J. (2008). First report of Simplicillium lanosoniveum causing brown spot on Salvinia auriculata and S. molesta in Taiwan. Plant disease, 92(11), 1589. doi: 10.1094/PDIS-92-11-1589C

Dong, Q.L., Lin, T.Y., Xing, X.Y., Chen, B., Han, Y. (2013). Identification of a symbiotic fungus from blue–green alga and its extracellular polysaccharide. Lett. App. Microbiol., 58, 303-310. doi: 10.1111/lam.12192

Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res., 32(5), 1792-1797. doi: 10.1093/nar/gkh340

Kumar, S., Stecher, G., Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol. Biol. Evol., 33, 1870-1874. doi: 10.1093/molbev/msw054

Lim, S.Y., Lee, S., Kong, H.G., Lee, J. (2014). Entomopathogenicity of Simplicillium lanosoniveum isolated in Korea. Mycobiology, 42(4), 317-321. doi: 10.5941/MYCO.2014.42.4.317

Liu, W., Xie, Y., Xue, J., Gao, Y., Zang, Y., Zhang, X., Tan, J. (2009). Histopathological changes of Ceroplastes japonicus infected by Lecanicillium lecanii. J. Invertebr. Pathol., 101, 96-105. doi: 10.1016/j.jip.2009.03.002

Lockwood, D.A. (1993). Environmental issues involved in biological control of rangeland grasshopper (Orthoptera: Acrididae) with exotic agents. Environ. Entomology, 22, 503-518. doi: 10.1093/ee/22.3.503

Saitou, N., Nei, M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol., 4, 406-425. doi: 10.1093/oxfordjournals.molbev.a040454

Wang, N., Xie, Y., Fan, J. (2016). Pathogenicity of Simplicillium lanosoniveum TYL001 isolated from Pseudaulacaspis pentagona. Mycosystema, 35(5), 559‐568.

Ward, N.A., Schneider, R.W., Aime, M.C. Colonization of soybean rust sori by Simplicillium lanosoniveum. Fungal Ecol., 4, 303-308. doi: 10.1016/j.funeco.2011.03.008

White, T.J., Bruns, T., Lee, S., Taylor, J. (1990). Amplification and direct sequencing of fungi ribosomal RNA genes for phylogenetics. In M.A. Innis, D.H. Gelfand, J.J. Sninsky and T.J. White (Eds.), PCR Protocols. A Guide to Methods and Applications (pp. 315-322). San Diego: Academic Press.

Zhao, D., Liu, B., Li, L.Y., Zhu, X.F., Wang, Y.Y., Wang, J.Q., Duan, Y.X., Chen, L.J. (2013). Simplicillium chinense: a biological control agent against plant parasitic nematodes. Biocontrol Sci. Techn., 23, 980-986. doi: 10.1080/09583157.2013.809514




DOI: http://dx.doi.org/10.15421/2017_1801

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.




Since April 2018 Journal changed the editorial policy and starts to be published exclusively in English, and changed its main site into www.ujecology.com

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

© 2017 Ukrainian Journal of Ecology. ISSN 2520-2138