Some properties of Bacillus subtilis probiotic supplements from byproducts of agricultural complex

N. A. Ushakova, R. V. Nekrasov, E. S. Brodskiy, V. V. Voznesenskaya, A. A. Kozlova, V. A. Samoylenko, M. G. Chabaev, E. K. Tomgorova, A. A. Zelenchenkova


Previously, it was shown that B. subtilis B-8130 produce bactericides and biologically active substances while fermenting different phyto-substrates. The bacilli release somatostatin-like peptide during solid-state fermentation of beet pulp sugar, sea-buckthorn leaves and flax seeds. The growth of bacterial culture is accompanied by the formation of biofilm that encapsulates phyto-carriers. The combination of all above factors determines high biological activity of probiotics and their effects on digestion in animals. We tested the addition of 0.1% B. subtilis B-8130 fermented substrate to the pig ration. The experiments showed higher weight gain, lowered daily feed expenses and improved survival. We found substantial changes in morphology of the small intestine epithelial cells associated with intensive absorption of feed nutrients in pigs that were fed with fermented sea-buckthorn leaves. This suggests influence of probiotics on digestion mediated by hormonal system.


probiotic; bacillus; solid-state fermentation; phyto-substrate; somatostatin-like peptide; biofilm

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