Prooxidant-antioxidant balance in the organism of bulls (young cattle) after using cadmium load

B. Gutyj, V. Stybel, L. Darmohray, Y. Lavryshyn, I. Turko, Y. Hachak, A. Shcherbatyy, I. Bushueva, V. Parchenko, A. Kaplaushenko, O. Krushelnytska

Abstract


The results of studies of the effect of Cadmium influence on the state of the enzyme and nonenzyme system of antioxidant protection of young cattle organism, namely on the activity of superoxide dismutase, catalase, glutathione peroxidase, level of reduced glutathione, Selenium, vitamins A and E. It was determined that the feeding of young bulls with cadmium chloride in a dose of 0.04 mg / kg of body weight contributed to a decrease both, enzyme and non enzyme level of the system of antioxidant protection (superoxide dismutase by 22%, catalase - by 12%, renewed glutathione by 11%, glutathione peroxidase by 22 %, Selenium - by 18%, vitamin A - by 23%, vitamin E - by 27%,). The lowest level of the antioxidant protection system in the blood of young cattle was set at the sixteenth and twenty-fourth day of the experiment, which is due to the increased activation of lipoperoxidation processes and a disturbance of the balance between the activity of the antioxidant system and the intensity of peroxidation of lipids. The toxic effect of Cadmium contributes to the change in the stationary concentrations of the radical metabolites O2, ˙OH, NO2, which, in their turn, initiate the processes of peroxide oxidation of lipids, indicating an increase in the level of diene conjugates and malonic dialdehyde. The highest level of intermediate and final products of peroxid oxidation of lipids in the blood of young cattle was determined on the thirtieth day of the experiment, while comparing with the control group, they increased by 31 and 27%, respectively.


Keywords


cadmium; superoxide dismutase; catalase; glutathione peroxidase; recovered glutathione; vitamins; selenium

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References


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DOI: http://dx.doi.org/10.15421/2017_165

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