Determination of the content of antibiotics and sulfanilamide drugs in milk by screening method

E. V. Bayer, Yu. N. Novozhitskaya, L. V. Shevchenko, V. M. Mykhalska

Abstract


On the basis of the conducted research, an assessment of the suitability of the method for the determination of sulfanilamide preparations and antibiotics in milk by raw screening method was developed and conducted with the help of RX / MS / MS. It has been confirmed that a method based on liquid chromatography using a mass spectrometric detector (PC / MS / MS) is more sensitive and selective. The study was conducted in accordance with the "Plan for monitoring the residues of veterinary drugs and other pollutants in live and unprocessed food of animal origin" approved in Ukraine in accordance with Directive 96/23 / EC dated April 29, 1996. It is proved that the adapted method for the determination of sulfanilamide preparations and antibiotics is sensitive and according to its parameters meets the European requirements. This method is fast enough, practical and universal, since it allows simultaneous examination of about 70 antibacterial drugs at the same time in milk. It has been established that the method of determining the residual amount of sulfanilamide preparations and antibiotics by the PX / MS / MS method is suitable for the investigation of products of animal origin on the content of the following drugs: sulfaguanidine, sulfacetamide, sulfapiridine, sulfadiazinum, sulfamethoxazole, sulfatyazole, sulfamethazine, sulfamethizole, sulfabenzamide, sulfametazine, sulfhinkosolin, sulfadoxine, sulfadimetoxin, sulfanilamide, trimethoprim, sulfamonomethoxin, sulfamethoxypyridazine, sulfachloropyridazine, sulfamoxol, dapsone, amoxicillin, ampicillin, penicillin G, penicillin V, oxacillin, cloxacillin, naftsilin, dicloxacillin, tsefalonium, tsefozolin, cefoperazone, tsefkvinom, tsefopirin, tseftiofur, cephalexin, norfloxacin, enrofloxacin, ciprofloxacin, marbofloksatsyn, dyfloksatsyn, danofloksatsyn, sarafloksatsyn, flumekin, oksolinovuyu acid, nalidixic acid, oxytetracycline, chlortetracycline, tetracycline, doxycycline, epioxytetracycline, epioxytetracycline, epichlortetracycline, streptomycin, dihydrostreptomycin, gentamicin, kanamycin, apramicin, paromomycin, lincomits, Spectinomycin, tilmykozyn, erythromycin, yozamitsyn, spiramycin, tilozyn, tiamulin, colistin, hamitromitsyn, tulatromitsyn. In assessing the suitability of the method, the MS / MS detection parameters are set and the validation characteristics are determined.


Keywords


antibiotics; liquid chromatography method with MS-MS detector; estimation of the suitability of the method

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References


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DOI: http://dx.doi.org/10.15421/2017_163

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