ЕFFECT OF PLANT GROWTH REGULATORS ON ANTIOXIDANT ENZYMES PROTECTION UNDER ANTHROPOGENIC EMISSIONS

O. M. Vasilyuk

Abstract


The paper presents analysis of general (GА, Px, ΔЕ, ml∙sec) and specific (SA, Px, ΔЕ, mg∙sec) of oxidoreductase enzyme activity (Peroxidase, Px, EC 1.11.1.7), which together with Catalase (Cat, EC 1.11.1.6) forms a double link antioxidant protection of cells in the organisms of different levels of organization (non-specific response to stress) in the conditions of increasing redox processes under the exogenous stress. These markers of changeable environmental factors were sampled in the leaves of Salix alba L., which grew along Mokra Sura River (anthropogenically polluted with high level of salinity, experiment) and Shpakova River (without anthropogenic impact, control). These rivers belong to the basin of the Dnieper River (Steppe Dnieper region) that has high industrial load. We used plant growth regulator "Kornevin" in order to accelerate the rooting and reduce the exogenous pressures on the plants. We detected nonspecific reaction towards peroxidase in anthropogenic pressure conditions and determined significant differences between experiment and control regards antioxidant protection depending on growth and development conditions.

Keywords


Salix alba L.; level of salinity; growth regulators; peroxidase; adaptogene; anthropogenic factors

Full Text:

PDF

References


Azcón, R., Perálvarez, M.C., Biró, B., Roldan, A., Ruíz-Lozano, J.M. (2009). Antioxidant activities and metal acquisition in mycorrhizal plants growing in a heavy-metal multicontami-nated soil amended with treated lignocellulosic agrowaste. Appl. Soil Ecol. 41, 168–177.

Becerril, F.R., Juárez-Vázquez, L.V., Hernández-Cervantes, S.C., Acevedo-Sandoval, O.A., Vela-Correa, G., Cruz-Chávez, E., Moreno-Espíndola, I.P., Esquivel-Herrera, A., de León-González, F. (2013). Impacts of manganese mining activity on the environment: Interactions among soil, plants and arbuscular Mycorrhiza. Environ. Contam. Toxicol. 64(2), 219–227.

Bendaly, A., Messedi, D., Smaoui, A., Ksouri, R., Bouchereau, A. Chedly A. (2016). Physiological and leaf metabolome changes in the xerohalophyte species Atriplex halimus induced by salinity, Plant Physiology and Biochemistry, 208-218.

Boshoff, М., Jonge, М.De., Scheifler, R., Bervoets, L. (2014). Predicting As, Cd, Cu, Pb and Zn levels in grasses (Agrostis sp. and Poa sp.) and stinging nettle (Urtica dioica) applying soil–plant transfer models. Sci. Total. Environ. 493(15), 862–871.

Deeba F., Pandey A.K., Ranjan S, Mishra A, Singh R, Sharma Y.K, Shirke P.A, Pandey V (2012). Physiological and proteomic responses of cotton (Gossypium herbaceum L.) to drought stress. 53, 6-18.

Fang, F., Zhang, X.-lian., Luo, H.-hui, Zhou, J.-jian, Gong, Y.-hui, Li, W.-jun, Shi, Zh.-wan, He, Q., Wu, Q., Li, L., Jiang, L.-, Cai, Zh.-gao, Oren-Shamir, M., Zhang, Zh.-qi, Pang. X.-qun. (2015). An intracellular laccase is responsible for eplicatechin-Mediated Anthocyanin degradation in Litci gruit pericarp. Plant Physiol. 169(4): 2391-2408. doi: 10.1104/pp.15.00359.

Fuller-Espie, S.L., Bearoff, F.M., Minutillo, M.A. (2011). Exposure of coelomocytes from the earthworm Eisenia hortensis to Cu, Cd, and dimethylbenz[a]anthracene: An in vitro study examining reactive oxygen species production and immune response inhibition. Pedobiologia, 54(29), S31–S36.

Hameed, A., Mahmooduzzafar, T.N.Q, Siddiqi, T.O., Iqbal, M. (2011). Differential activation of the enzymatic antioxidant system of Abelmoschus esculentus L. under CdCl2 and HgCl2 exposure. Braz. J. Plant Physiol, 23(1), 46–54.

Hasan, S.A., Hayat, S., Wani, A.S, Ahmad, A. (2011). Establishment of sensitive and resistant variety of tomato on the basis of photosynthesis and antioxidative enzymes in the presence of cobalt applied as shotgun approach. Braz. J. Plant Physiol, 23(3), 175–185.

Kumar М., Bijo A. J., Baghel R.S., Reddy С.R.K., Jha B. (2012). Selenium and spermine alleviate cadmium induced toxicity in the red seaweed Gracilaria dura by regulating antioxidants and DNA methylation, 51, 129-138.

Kuta, E., Jędrzejczyk-Korycińska, M., Cieślak, E., Rostański, A, Szczepaniak, M., Migdałek, G, Li, Z.H., Chen, L., Wu, Y.H., Li, P. (2014). Effects of mercury on oxidative sand gene expression of potential biomarkers in larvae of the Chinese rare minnow Gobiocypris rarus. Environ. Contam. Toxicol, 67(2), 245–251.

Li, Zh.-H., Chen., L., Wu, Y-H., g Li., P., Li, Y.-F., Ni, Zh.-H. (2014). Effects of Mercury on Oxidative Stress and Gene Expression of Potential Biomarkers in Larvae of the Chinese Rare Minnow Gobiocypris Rarus. Archives of Environmental Contamination and Toxicology, 67(2), 245–251.

Mohammadian, M.A., Mobrami, Z., Sajedi, R.H. (2011). Bioactive compounds and antioxidant capacities in the flavedo tissue of two citrus cultivars under low temperature. Braz. J. Plant Physiol, 23(3), 203-208.

Mohamed, A.A., Castagna, A., Ranieri, A., di Toppi, S. (2012). Cadmium tolerance in Brassica juncea roots and shoots is affected by antioxidant status and phytochelatin biosynthesis. Plant Physiology and Biochemistry, 57, 15-22.

Naji, K.M., Devaraj, V.R. (2011). Antioxidant and other biochemical defense responses of Macrotyloma uniflorum (Lam.) Verdc. (Horse gram) induced by high temperature and salt stress. Braz. J. Plant Physiol, 23(3), 187-195

Ragel, P., Ródenas, R., García-Martín, E., Andrés, Z., Villalta, I., Nieves-Cordones, M., Rivero, Rosa M., Martínez, V., Pardo, Jose M., Quintero, Francisco J., Rubio, F. (2015). The CBL-Interacting Protein Kinase CIPK23 Regulates HAK5-Mediated High-Affinity K+ Uptake in Arabidopsis Roots. Plant Physiol, 169(4): 2863-2873. doi: 10.1104/pp.15.00359.

Raggi, S., Ferrarini, A., Delledonne, M., Dunand, C., Ranocha, P., De Lorenzo, G., Cervone, F., Ferrari, S. (2015). The Arabidopsis Class III Peroxidase AtPRX71 Negatively Regulates Growth under Physiological Conditions and in Response to Cell Wall Damage. Plant Physiol, 169(4):2513-2525. doi: 10.1104/pp.15.01464.

Sidhu, G.P.S., Singh, H.P., Batish, D.R., Kohli, R.K. (2016). Effect of lead on oxidative status, antioxidative response and metal accumulation in Coronopus didymus. Plant Physiology and Biochemistry, 105, 290-296.

Thounaojam, T.C., Panda, P., Mazumdar, P., Kumar, D., Sharma, G.D., Sahoo, L., Panda, S.K. (2012). Excess copper induced oxidative stress and response of antioxidants in rice. Plant Physiology and Biochemistry, 53, 33-39.

Vasilyuk, O.M., Pakhomov, A.E. (2014). Еffect of lead ions on Alanine Aminotransferase activity in Glechoma hederacea leaves subject. Scientific Enquiry in the Contemporary World: Theoretical Basics and Innovative Approach. Ser. Natural sciences. B&M Publishing San Francisco, California, USA, B&M Publishing Research and Publishing Center “Colloquium”, 1, 19–26.

Vasilyuk, O.M., Dzyubak, O.I. (2009). Physiological and biochemical parameters of plants as markers of a condition of environment // Матеріали І міжнародної наукової конференції студентів, аспірантів та молодих вчених «Фундаментальні та прикладні дослідження в біології», том ІІ, Донецьк, «Вебер», – С. 348–349.

Van Emden, H.F. 2008. Statistics for terrified biologists. Blackwell, Oxford.

Vestena, S., Cambraia, J., Ribeiro, C, Oliveira, J.A. Oliva, M.A. (2011). Cadmium induced oxidative stress and antioxi-dative enzyme response in water hyacinth and salvinia. Braz. J. Plant Physiol. 23(2), 131-139.

Zhang, Y., De Stefano, R., Robine, M., Butelli, E., Bulling, K., Hill, L., Rejzek, M., Martin, C., Schoonbeek, H.-jan. (2015). Different Reactive Oxygen Species Scavenging Properties of Flavonoids Determine Their Abilities to Extend the Shelf Life of Tomato. Plant Physiol. 169(3): 1568-1583 doi: 10.1104/pp.15.00346.

Варфоломеев С.Д. Химическая ензимология. М: «Академия", 2005.-580 с.

Василюк, О.М. Вплив засолення на біохімічні показники в листках Salix alba L. на фоні дії регуляторів росту рослин гумінової природи Текст / О.М. Василюк // Матеріали Міжнародної науково-практичної конференції «Біорізноманіття: теорія, практика та методичні аспекти вивчення у загальноосвітній та вищій школі», Полтава, 2010. – С.54–56.

Василюк, О.М. Вплив іонів свинцю та малих доз радіації на активність каталази у проростках кукурудзи / О.М. Василюк, О.М. Вінниченко // Вісн. Дніпропетр. унів. Сер. Біол. Екол. – 2006а, 14(2). – С. 30–33.

Василюк, О.М. Вплив біологічно активних речовин на активність каталази кукурудзи різних генотипів на фоні дії аценіту / О.М. Василюк., О.М. Вінниченко // Вісн. Дніпропетр. унів. Сер. Біол. Екол. – 2006b. – №3/1. – С.26–30.

Василюк, О.М. Вплив регуляторів росту на активність ферментів переамінування в листі та коренях Salix alba L. / О.М. Василюк, П.В. Гриценко // Вісн. Дніпропетр. унів. Сер. Біол. Екол. – 2008.– 16(1). – С.34-40.

Верхотуров, В.В. Влияние перекиси водорода на анти- и прооксидантный статус семян ячменя при прорастании / В.В. Верхотуров, В.К. Франтенко // Доклады Российской академии с/х наук, 2008. – № 1. – С. 11–13.

Виноградова, Е.Н. Устойчивость пероксидазы из листьев растений техногенних экотопов к действию ингредиентов эмиссий в условиях in vitro / Е.Н. Виноградова // Факторы устойчивости растений в экстремальных природных условиях и техногенной среде. Коллектив авторов. – М. – Берлин: Директ-Медиа, 2015. – 501с.

Дзюбак, О.І. Василюк, О.М. Вплив хлоридного засолення на морфометричні та біохімічні показники рослин у динаміці росту та розвитку / О.І. Дзюбак, О.М. Василюк // Матеріали І міжнародної наукової конференції студентів, аспірантів та молодих вчених «Фундаментальні та прикладні дослідження в біології», том ІІ, Донецьк, «Вебер», 2009. – С. 231–232.

Дерендовская, А.И. Особенности изоферментного спектра пероксидаз листьев у подвойных сортов и привойно-подвойных комбинаций винограда. / А.И. Дерендовская, А.В. Штирбу // Lucrări şt., Univ. Agrară de Stat din Moldova. 2011, Vol. 29: Agronomie, Р. 103–107.

Скворцов, А.К. Ивы СССР / А.К. Скворцов. — М.: "Наука". —1968. — 262 с.

Чупахина Г.Н. Физиологические и биохимические методы анализа растений. – Калининград, 2000. –59 с.

Шевелюха, В.С. Регуляторы роста растений Текст / В.С.

Шевелюха. – M.: Агропромиздат, 1990. – 185с.

REFERENCES

Azcón, R., Perálvarez, M.C., Biró, B., Roldan, A., Ruíz-Lozano, J.M. (2009). Antioxidant activities and metal acquisition in mycorrhizal plants growing in a heavy-metal multicontami-nated soil amended with treated lignocellulosic agrowaste. Appl. Soil Ecol,41, 168–177.

Becerril, F.R., Juárez-Vázquez, L.V., Hernández-Cervantes, S.C., Acevedo-Sandoval, O.A., Vela-Correa, G., Cruz-Chávez, E., Moreno-Espíndola, I.P., Esquivel-Herrera, A., de León-González, F. (2013). Impacts of manganese mining activity on the environment: Interactions among soil, plants and arbuscular Mycorrhiza. Environ. Contam. Toxicol, 64(2), 219–227.

Bendaly, A., Messedi, D., Smaoui, A., Ksouri, R., Bouchereau, A. Chedly A. (2016). Physiological and leaf metabolome changes in the xerohalophyte species Atriplex halimus induced by salinity, Plant Physiology and Biochemistry, 208-218.

Boshoff, М., Jonge, М.De., Scheifler, R., Bervoets, L. (2014). Predicting As, Cd, Cu, Pb and Zn levels in grasses (Agrostis sp. and Poa sp.) and stinging nettle (Urtica dioica) applying soil–plant transfer models. Sci. Total. Environ, 493(15), 862–871.

Chupahina, G.N. (2000). Physiological and biochemical analysis methods rasteniy. Kaliningrad (in Russian).

Deeba, F., Pandey, A.K., Ranjan, S., Mishra, A., Singh, R., Sharma, Y.K., Shirke, P.A, Pandey, V. (2012). Physiological and proteomic responses of cotton (Gossypium herbaceum L.) to drought stress. Plant Physiol Biochem, 53, 6-18.

Derendovskaya, A.I., Ştirbu, A.V. (2011). Osobennosti izofermentnogo spektra peroksidaz listev u podvoynyih sortov i privoyno-podvoynyih kombinatsiy vinograda. Lucrări şt, Univ. Agrară de Stat din Moldova. Agronomie, 29, 103–107 (in Russian).

Dzyubak, O.I., Vasilyuk, O.M. (2009). Effect of chloride salinity on morphometric and biochemical indices in the dynamics of plant growth and development. Proceed. I International Scientific Conference Fundamental and applied research in biology. Donetsk: Veber.

Fang, F., Zhang, X.-lian., Luo, H.-hui, Zhou, J.-jian, Gong, Y.-hui, Li, W.-jun, Shi, Zh.-wan, He, Q., Wu, Q., Li, L., Jiang, L., Cai, Zh.-gao, Oren-Shamir, M., Zhang, Zh.-qi, Pang. X.-qun. (2015). An intracellular laccase is responsible for eplicatechin-Mediated Anthocyanin degradation in Litci gruit pericarp. Plant Physiol, 169(4): 2391-2408 doi: 10.1104/pp.15.00359.

Fuller-Espie, S.L., Bearoff, F.M., Minutillo, M.A. (2011). Exposure of coelomocytes from the earthworm Eisenia hortensis to Cu, Cd, and dimethylbenz[a]anthracene: An in vitro study examining reactive oxygen species production and immune response inhibition. Pedobiologia, 54(29), 31–36.

Hameed, A., Mahmooduzzafar, T.N.Q, Siddiqi, T.O., Iqbal, M. (2011). Differential activation of the enzymatic antioxidant system of Abelmoschus esculentus L. under CdCl2 and HgCl2 exposure. Braz. J. Plant Physiol, 23(1), 46–54.

Hasan, S.A., Hayat, S., Wani, A.S, Ahmad, A. (2011). Establishment of sensitive and resistant variety of tomato on the basis of photosynthesis and antioxidative enzymes in the presence of cobalt applied as shotgun approach. Braz. J. Plant Physiol, 23(3), 175–185.

Kumar, М., Bijo, A. J., Baghel, R.S., Reddy, С.R.K., Jha, B. (2012). Selenium and spermine alleviate cadmium induced toxicity in the red seaweed Gracilaria dura by regulating antioxidants and DNA methylation. Plant Physiol Biochem, 51, 129-138.

Kuta, E., Jędrzejczyk-Korycińska, M., Cieślak, E., Rostański, A, Szczepaniak, M., Migdałek, G, Li, Z.H., Chen, L., Wu, Y.H., Li, P. (2014). Effects of mercury on oxidative sand gene expression of potential biomarkers in larvae of the Chinese rare minnow Gobiocypris rarus. Environ. Contam. Toxicol, 67(2), 245–251.

Li, Zh.-H., Chen, L., Wu, Y-H., Li, P., Li, Y.-F., Ni, Zh.-H. (2014). Effects of Mercury on Oxidative Stress and Gene Expression of Potential Biomarkers in Larvae of the Chinese Rare Minnow Gobiocypris Rarus. Archives of Environmental Contamination and Toxicology, 67(2), 245–251.

Mohammadian, M.A., Mobrami, Z., Sajedi, R.H. (2011). Bioactive compounds and antioxidant capacities in the flavedo tissue of two citrus cultivars under low temperature. Braz. J. Plant Physiol, 23(3), 203-208.

Mohamed, A.A., Castagna, A., Ranieri, A., di Toppi, S. (2012). Cadmium tolerance in Brassica juncea roots and shoots is affected by antioxidant status and phytochelatin biosynthesis. Plant Physiology and Biochemistry, 57, 15-22.

Naji, K.M., Devaraj, V.R. (2011). Antioxidant and other biochemical defense responses of Macrotyloma uniflorum (Lam.) Verdc. (Horse gram) induced by high temperature and salt stress. Braz. J. Plant Physiol, 23(3), 187-195.

Ragel, P., Ródenas, R., García-Martín, E., Andrés, Z., Villalta, I., Nieves-Cordones, M., Rivero, Rosa M., Martínez, V., Pardo, Jose M., Quintero, Francisco J., Rubio, F. (2015). The CBL-Interacting Protein Kinase CIPK23 Regulates HAK5-Mediated High-Affinity K+ Uptake in Arabidopsis Roots. Plant Physiol, 169(4): 2863-2873. doi: 10.1104/pp.15.00359.

Raggi, S., Ferrarini, A., Delledonne, M., Dunand, C., Ranocha, P., De Lorenzo, G., Cervone, F., Ferrari, S. (2105). The Arabidopsis Class III Peroxidase AtPRX71 Negatively Regulates Growth under Physiological Conditions and in Response to Cell Wall Damage. Plant Physiol, 169(4):2513-2525. doi: 10.1104/pp.15.01464.

Shevelukha, V.S. (1990). Regulyatoryi rosta rasteniy. Moscow: Agroprom Press (in Russian).

Sidhu, G.P.S., Singh, H.P., Batish, D.R., Kohli, R.K. (2016). Effect of lead on oxidative status, antioxidative response and metal accumulation in Coronopus didymus. Plant Physiology and Biochemistry, 105, 290-296.

Skvortsov, A.K. (1968). Іvyi SSSR. Мoscow: Nauka (in Russian).

Thounaojam, T.C., Panda, P., Mazumdar, P., Kumar, D., Sharma, G.D., Sahoo, L., Panda, S.K. (2012). Excess copper induced oxidative stress and response of antioxidants in rice. Plant Physiol Biochem, 53, 33-39.

Van Emden, H.F. (2008). Statistics for terrified biologists. Blackwell, Oxford.

Varfolomeyev, S.D. (2005). Chemical enzymology. Moscow: Academy (in Russian).

Vasilyuk, O.M. (2010). Effect of plant growth regulators of humic nature on biochemical parameters in leaves of Salix alba L. in condition of water salinity. Proceed. International Scientific and Practical Conference Biodiversity: theory, practice and methodological aspects of studying in secondary and high school. Poltava (in Russian).

Vasilyuk, O.M., Dzyubak, O.I. (2009). Physiological and biochemical parameters of plants as markers of a condition of environment. Proceed. I International scientific conference of students and young scientists Fundamental and applied research in biology. Donetsk: Veber.

Vasilyuk, O.M., Gritsenko, P.V. (2008). Vpliv regulyatoriv rostu na aktivnist fermentiv pereaminuvannya v listi ta korenyah Salix alba L. Visn. Dnipropetr. Univ. Ser. Biol. Ekol., 16(1), 34–40 (in Ukrainian).

Vasilyuk, O.M., Pakhomov, A.E. (2014). Еffect of lead ions on Alanine Aminotransferase activity in Glechoma hederacea leaves subject. Scientific Enquiry in the Contemporary World: Theoretical Basics and Innovative Approach. Ser. Natural sciences. B&M Publishing San Francisco, California, USA, B&M Publishing Research and Publishing Center “Colloquium”, 1, 19–26.

Vasilyuk, O.M., Vinnichenko, O.M. (2006a). Vpliv іonіv svintsyu ta malih doz radіatsіyi na aktivnist katalazi u prorostkah kukurudzi. Visn. Dnipropetr. Univ. Ser. Biol. Ekol., 14(2), 30–33 (in Ukrainian).

Vasilyuk, O.M., Vinnichenko, O.M. (2006b). Vpliv biologichno aktivnih rechovin na aktivnist katalazi kukurudzi riznih genotipiv na foni diyi atsenitu. Visn. Dnipropetr. Univ. Ser. Biol. Ekol., 14(1), 26–30 (in Ukrainian).

Verkhoturov, V.V., Frantenko, V.K. (2008). Vliyanie perekisi vodoroda na anti- i prooksidantnyiy status semyan yachmenya pri prorastanii. Reports of the Russian Agricultural Science Academy, 1, 11–13 (in Russian).

Vestena, S., Cambraia, J., Ribeiro, C, Oliveira, J.A. Oliva, M.A. (2011). Cadmium induced oxidative stress and antioxi-dative enzyme response in water hyacinth and salvinia. Braz. J. Plant Physiol, 23(2), 131-139.

Vinogradova, E.N. (2015). Ustoychivost peroksidazyi iz listev rasteniy tehnogennih ekotopov k deystviyu ingredientov emissiy v usloviyah in vitro. Factors of plant resistance to extreme environmental conditions and man-made environment. Moscow, Berlin: Direkt-Media (in Russian).

Zhang, Y., De Stefano, R., Robine, M., Butelli, E., Bulling, K., Hill, L., Rejzek, M., Martin, C., Schoonbeek, H.-jan. (2015). Different Reactive Oxygen Species Scavenging Properties of Flavonoids Determine Their Abilities to Extend the Shelf Life of Tomato. Plant Physiol, 169(3), 1568-1583 doi: 10.1104/pp.15.00346.




DOI: http://dx.doi.org/10.15421/201674

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.




Since April 2018 Journal changed the editorial policy and starts to be published exclusively in English, and changed its main site into www.ujecology.com

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

© 2017 Ukrainian Journal of Ecology. ISSN 2520-2138