Antimicrobial properties of Tanacetum balsamita L. (Asteraceae) introduced in Ukrainian Polissya

I. V. Ivashchenko

Abstract


The paper studies antimicrobial activity of ethanol extract of Tanacetum balsamita L. from the family Asteraceae, tribus Anthemideae, the plant being medicinally and pharmaceutically promising. Antimicrobial properties of Tanacetum balsamita were studied on test-cultures, collected from the Ukrainian Collection of Microorganisms (UCM, Institute of microbiology and virology SAS Ukraine), the test-cultures being of Escherichia coli (coliform bacillius) UCM B-906 (ATCC 25922); Staphilococcus aureus (golden staphylococcus) UCM B-904 (ATCC 25923); Pseudomonas aeruginosa (blue pus bacillus) UCM B-900 (ATCC 9027); Candida albicans UCM Y-1918 (ATCC 885-653). The above microorganisms are test strains used to determine antimicrobial properties of medicines. The antimicrobial effect of the investigated substances was studied by the method of successive dilutions which determines minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). The extract has demonstrated selective antimicrobial activity against gram-positive bacteria strains of Staphylococcus aureus. In comparison with the solvent, MIC and MBC values showed an 8-times increase. T. balsamita extract revealed no bacteriostatic, bacteriocidal/fugicidal effect against gram-negative bacteria stains of Escherichia coli, P. aeruginosa and fungus C. albicans. The antimicrobial activity of the extract against S. aureus may be attributed to the presence of essential oil and phenolic compounds. The major compounds in the essential oil are epoxylynaloolacetate (60.13 %) and b-thujone (29.7 %). The following phenolic compounds have been determined with the high-performance liquid chromatography method: flavonoids – luteolin, apigenin, diosmetin, acacetin, apigenin-7-bioside, acacetin-7-bioside, apigenin-7-glycoside, luteolin-7-glycoside, diosmetin-7-bioside, diosmetin-7-glycoside, diosmetin-7-rhamnoside, and hydroxycinnamic acids – n-coumaric, caffeic, chlorogenic, isochlorogenic. The dominant compound is isochlorogenic acid (35 % of the total phenolic compounds detected). The experimental data obtained gives evidence that further research and application of T. balsamita is promising for creation of new anti-staphylococcus drugs.


Keywords


Tanacetum balsamita L.; Asteraceae; introduction; extract; microorganisms; antimicrobial activity.

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References


Гулько Р. М. Словник лікарських рослин світової медицини / Р. М. Гулько. – Львів: Ліга-Прес, 2005. – 506 с.

Іващенко І. В. Хроматографічний аналіз фенольних сполук Tanacetum balsamita L. за умов інтродукції в Житомирському Поліссі / І. В. Іващенко // Физиология растений и генетика. – 2016. – Т. 48, № 2. – С. 178 – 183.

Кухарева Л. В. Пиретрум бальзамический – Pyrethrum balsamita (L.) Willd. в условиях Беларуси / Л. В. Кухарева, И. Н. Тычина, И. М. Савич // Анализ и прогнозирование результатов интродукции декоративных и лекарственных растений мировой флоры в ботанические сады : материалы 2-й Международной конференции, г. Минск, 26-28 августа. – Минск: Тэхналогія, 1996. – С. 126.

Машенцева А. А. Експериментальное и теоретическое исследование взаимосвязи «структура-активность» производных коричной кислоты / А. А. Машенцева, Т. С. Сейтенбетов // Journal of Siberian Federal University. Chemistry. – 2010. – №3. – P. 183 – 192.

Мінарченко В. М. Лікарські судинні рослини України / В. М. Мінарченко. – Київ: Фітосоціоцентр, 2005. – 324 с.

Миллер Д. Эксперименты в молекулярной генетике / Д. Миллер [ред. С. И. Алиханяна]. – М.: Мир, 1976. – С. 394 – 395.

Про затвердження методичних вказівок «Визначення чутливості мікроорганізмів до антибактеріальних препаратів»: Наказ МОЗ України №167. – [Чинний від 2007-04-05]. – К.: МОЗ України, 2007. – 63с.

Украинская коллекция микроорганизмов. Каталог культур / под ред. В. С. Подгорского, О. И. Коцофляк, Е. А. Киприановой, О. Р. Гвоздяк. – К.: Наукова думка, 2007. – 270 с.

Alexieva I., Mihaylova D., Popova A. Evaluation of the antioxidant capacity of aqueous extracts of fresh Chrysanthemum balsamita L. leaves growing in Bulgaria // Научни трудове на русенския університет. – 2013. – 52, cерия 10.2. – С. 88 – 91.

Bylaite E. Composition of essential oil of costmary [Balsamita major (L.) desf.] at different growth phases / Е. Bylaite, R. Venscutonis, J. P. Roozen, M. A. Posthumus // J. Agricult. Food Chem. – 2000. – 48(6). – P. 2409 – 2414.

Essential Oils in Food Preservation, Flavor and Safety / V. Preedy (ed.) – London: Academic Press, 2016. – 930 p. doi:10.1016/B978-0-12-416641-7.00041-9

Gallori S. Chemical composition of some traditional herbal drug preparations: Essential oil and aromatic water of costmary (Balsamita suaveolens Pers. ) / S. Gallori, G. Flamini, A. R. Bilia, I. Morelli, A. Landini, F. F. Vincieri // J. Agric. Food Chem. - 2001. – 49. – P. 5907 – 5910.

Hassanpouraghdam M. B. Chrysnthemum Balsamita (L.) Baill.: a Forgotten Medicinal Plant / M. B. Hassanpouraghdam, S. J. Tabatabaie, H. Nazemiyeh, L. Vojodi, M. A. Aazami, A. M. ShoJa // Facta Universitatis Series: Medicine and Biology. – 2008. – 15, № 3. – P. 119 – 124.

Hassanpouraghdam M. B., Tabatabaie S. J., Nazemiyen H., Vojodi L. Volatile oil constituents of alecost [Tanacetum balsamita L. ssp. Balsamitoides (Schults-Bip.)] growing wild in North-West of Iran. // Herba polonica. – 2009. – 55, № 1. – P. 53 – 59.

Ivashchenko I. V. The Essential Oils Composition of Tanacetum balsamita var. tanacetoides Boiss. and Tanacetum balsamita var. balsamitoides ( Sch. Bip) P. D. Sell under the Condition of their introduction in the Ukrainian Polissya / I. V. Ivashchenko, O. A. Ivashchenko, D. B. Rakhmetov // Agrobiodiversity for improving nutrition, health and life quality Part I. – Nitra: Slovak University of Agriculture in Nitra, 2015. – P. 293 – 297.

Lorestani F. A. Fumigant toxicity of essential oil from Tanacetum balsamita L. (Compositae) against adults and eggs of Callosobruchus maculatus F. (Coleoptera: Bruchidae) / F. A. Lorestani, A. Khashaveh, R. A. Lorestani // Archives Of Phytopathology And Plant Protection. – 2013. – Vol. 46, Issue 17. – P. 2080 – 2086. doi:10.1080/03235408.2013.785112.

Mărculescu, A. Contribution à l’étude clinique des taxons chimiques de l’espèce Chrysanthemum balsamita L. / А. Mărculescu // Phytothérapie. – 2013. – Vol. 11, Issue 4. – P. 219 – 224. doi:10.1007/s10298-013-0793-7

Nakatani N. Identification, quantitative determination and antioxidative activities of chlorogenic acid isomers in prune (Prunus domestica L.) / N. Nakatani, S. Kayano, H. Kikuzaki, K. Sumino, K. Katagiri, T. Mitani // J. Agric. Food Chem. – 2000. – 48. – P. 5512 – 5516.

Peana A. T. Anti-inflammatory activity of linalool and linalyl acetate constituents of essential oils /A. T. Peana, P. S. D'Aquila, F. Panin, G. Serra, P. Pippia, M. D. Moretti // Phytomedicine. – 2002. – 9(8). – 721 – 726. doi:10.1078/094471102321621322

Referenses

Alexieva, I., Mihaylova, D., Popova, A. (2013). Evaluation of the antioxidant capacity of aqueous extracts of fresh Chrysanthemum balsamita L. leaves growing in Bulgaria. Научни трудове на русенския університет. 52, (10.2.), 88–91.

Bylaite, E., Venscutonis, R., Roozen, J. P., Posthumus, M. A. (2000). Composition of essential oil of costmary [Balsamita major (L.) desf.] at different growth phases. J. Agricult. Food Chem, 48(6), 2409–2414.

Gallori, S., Flamini, G., Bilia, A. R., Morelli, I., Landini, A.,Vincieri, F. F. (2001). Chemical composition of some traditional herbal drug preparations: Essential oil and aromatic water of costmary (Balsamita suaveolens Pers.). J. Agric. Food Chem., 49, 5907–5910.

Hassanpouraghdam, M. B., Tabatabaie, S. J., Nazemiyeh, H., Vojodi, L., Aazami, M. A., ShoJa, A. M. (2008). Chrysnthemum Balsamita (L.) Baill.: a Forgotten Medicinal Plant. Facta Universitatis. Series: Medicine and Biology, 15 (3), 119–124.

Hassanpouraghdam, M. B., Tabatabaie, S. J., Nazemiyen, H., Vojodi, L. (2009). Volatile oil constituents of alecost [Tanacetum balsamita L. ssp. Balsamitoides (Schults-Bip.)] growing wild in North-West of Iran. Herba polonica, 55 (1), 53–59.

Hulko R. M. (2005). Dictionary of medicinal plants of the world medicine. Lviv: Liga-Press. (In Ukrainian)

Ivashchenko, I. V., Ivashchenko, O. A., Rakhmetov, D. B. (2015). The Essential Oils Composition of Tanacetum balsamita var. tanacetoides Boiss. and Tanacetum balsamita var. balsamitoides (Sch. Bip) P. D. Sell under the Condition of their introduction in the Ukrainian Polissya. Agrobiodiversity for improving nutrition, health and life quality Part I. Nitra: Slovak University of Agriculture in Nitra, 293–297.

Ivashchenko, I. V. (2016). Chromatographic analysis of phenolic compounds T. balsamita (Asteraceae) under the conditions of their introduction in the Zhytomir Polissya. Fiziol.rast.genet, 48(2),178–183.

Kuhareva, L. V., Tychina, I. N., Savich, I. M. (1996). Pyrethrum. balsamita (L.) Willd. Under conditions of Belarus. Analysis and prognostication of the results of introducing the world flora decorative and medicinal plants into botanical gardens: proceeding of the 2-nd International conference, Minsk, August, 26-28. Minsk: Technology. (In Russian)

Lorestani, F. A., Khashaveh, A., Lorestani, R. A. (2013). Fumigant toxicity of essential oil from Tanacetum balsamita L. (Compositae) against adults and eggs of Callosobruchus maculatus F. (Coleoptera: Bruchidae). Archives Of Phytopathology And Plant Protection, 46 (17), 2080 – 2086. doi:10.1080/03235408.2013.785112.

Mărculescu, A. (2013). Contribution à l’étude clinique des taxons chimiques de l’espèce Chrysanthemum balsamita L. Phytothérapie, 11 (4), 219 – 224. doi:10.1007/s10298-013-0793-7.

Mashentseva, A. A., Seitenbetov, T. S. (2010). Experimental and theoretical research of interrelation “structure-activity” of the cinnamic acid derivatives. Journal of Siberian Federal University. Chemistry, 3, 183–192. (In Russian)

Methodical instructions ‘Determination of the sensibility of microorganisms to antibacterial preparations’ (2007). The Order of the Ministry of Health of Ukraine No 167. Kyiv: Ministry of Health of Ukraine. (In Ukrainian)

Miller, D. Experiments in molecular genetics. (1976). Moscow: Mir Publishers. (In Russian)

Minarchenko, V. M. (2005). Medicinal vascular plants of Ukraine. Kyiv: Phytosociocentre. (In Ukrainian)

Nakatani, N., Kayano, S., Kikuzaki, H., Sumino, K., Katagiri, K., Mitani, T. (2000). Identification, quantitative determination and antioxidative activities of chlorogenic acid isomers in prune (Prunus domestica L.). J. Agric. Food Chem., 48, 5512–5516.

Peana, A. T., D'Aquila, P. S., Panin, F., Serra, G., Pippia, P., Moretti, M. D. (2002). Anti-inflammatory activity of linalool and linalyl acetate constituents of essential oils. Phytomedicine, 9(8), 721–726. doi:10.1078/094471102321621322

Podgorsky, V. S., Kotsoflyak, O. I., Kiprianova, Ye. I., Gvozdyak, O. R. The Ukrainian collection of microorganisms: Crop catalogue. (2007). (Eds.). Kyiv: Naukova Dumka Publishers. (In Russian)

Preedy, V. (2016). Essential Oils in Food Preservation, Flavor and Safety. London: Academic Press. doi:10.1016/B978-0-12-416641-7.00041-9.




DOI: http://dx.doi.org/10.15421/20176

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