ULTRASOUND-ENHANCED CELL PRODUCTION OF LACTIC AND PROPIONIC ACID BACTERIA UNDER SUBMERGED CULTIVATION FOR INDUSTRIAL PURPOSES

D. A. Durnikin, M. M. Silantyeva, O. V. Ereshchenko

Abstract


Lactic and propionic bacteria are actively used as feed and food biopreservatives. The industrial production of these bacteria is carried out using known standard biotechnological approaches and equipment. However, the modern requirements to the volumes of their production require the development of new technologies providing the more intensive growth of bacterial biomass. One of the possible ways to do it is the use of nonspecific stimulators of chemical or physical origin. The stimulating effect of ultrasound on live systems attracts attention of many researchers. Depending on the sonication parameters and conditions, the impact of ultrasound on cell cultures can either stimulate or suppress their life processes. The possibility of the ultrasound stimulation of the biomass accumulation process has been studied for submerged bacterial cultures of Lactococcus lactis VPKM B-2092, Lactobacillus plantarum VPKM B-4173, and Propionibacterium acidipropionici VPKM B-2092. The inoculum with cell contentration of 1 · 108 mL-1 was sonicated at 880 kHz and energy density varied within 0.1-0.7 W/cm3 using a specially designed cuvette, through which the cell suspension was introduced into a fermenter at a rate of 10 mL/s that provided the total sonication time equal to 100-120 seconds. As a signal source, a standard therapeutical ultrasound apparatus UZT-1.01F equipped with a sweep generator was used.

For all three cultures, the ultrasound stimulation resulted in a significant increase in the optical density of culture broth comparing to the control and the corresponding increase of the cell concentration. The optimum sonication energy density for the Lactococcus lactis VPKM B-2092, Lactobacillus plantarum VPKM B-4173, and Propionibacterium acidipropionici VPKM B-2092 was equal to 0.5, 0.3-0.5 и 0.3 W/cm3, respectively. Comparing to the control, the cell count of these strains in the culture broth increased in 28.6, 9, and 16.7 times, respectively.

Thus, the ultrasound stimulation of inoculum provides a significant increase in the biomass of cells producing lactic and propionic acid that, in turn, increases the economic efficiency of their industrial use. Since the mechanisms of such stimulating action of ultrasound are well-studied, and the exploitation of ultrasound generators is simple and cheap, the further development of the ultrasound stimulation approach seems to be very promising for the industrial microbiology.

 


Keywords


lactic acid bacteria; propionic acid bacteria; fermentation; stimulation; ultrasound

Full Text:

PDF

References


Акопян В.Б., Ершов Ю.А. Основы взаимодействия ультразвука с биологическими объектами (2-е изд.). – М.: ЮРАЙТ. – 2016. – 223 с.

Акопян В.Б., Коржевенко Г.Н., Шангин-Березовский Г.Н. Скрытый резерв роста и развития живых систем // Вестник сельскохозяйственной науки. – 1988. – № 4(380). – С. 96–105.

Акопян В.Б., Ступин А.Ю., Хабенский Б.М., Сергеева А.В. Мордвинова Е.М. Способ получения механозависимого фактора роста человека. Патент РФ № 2523908. – 2012.

ГОСТ Р МЭК 62127-1-2009. Общие требования к методам измерений и способам описания полей в частотном диапазоне от 0.5 до 40 МГц. М.: СтандартИнформ. – 2010. – 61 с.

Олешкевич А.А., Кутликова И.В., Черенкова И.А. Сравнительный анализ реакции разных видов лейкоцитов на обработку ультразвуком минимальной терапевтической интенсивности / Сборник научных трудов Международной научно-практической конференции «Теоретические и прикладные вопросы науки и образования». – Тамбов. – 2015. – Т. 1. – С. 107–111.

Шигаева М.Х. Разнообразие микроорганизмов // Вестник КазНУ. Серия биологическая. – 2002. – №1. – С. 133–137.

Chisti Y. Sonobioreactors: using ultrasound for enhanced microbial productivity // Trends in Biotechnology. – 2003. – V. 21(2). – P. 89–93.

Lye H.S., Khoo B.Y., Karim A.A., Rusul G., Liong M.T. Ultrasound enhanced growth and cholesterol removal of Lactobacillus fermentum FTDC 1311 in the parent cells but not the subsequent passages // Ultrasonics Sonochemistry. – 2012. – V. 19. – P. 901–908.

Oleshkevich A. Studies of frequency–dependent changes under modulated ultrasound exposure on cells in suspension // International Journal of Biomedicine. – 2015. – V. 4(1). – P. 30–34.

Tabatabaie F., Mortazavi S.A. Effects of ultrasound treatment on viability and autolysis of starter bacteria in hard cheese // American-Eurasian Journal of Agricultural & Environment Science. – 2010. – V. 8(3). – P. 301–304.

REFERENCES

Akopyan, V.B., Ershov, Yu.A. (2016). Osnovy vzaimodeistviya ultrazvuka s biologicheskimi ob’ektami. Moscow: YURAIT (in Russian).

Akopyan, V.B., Korzhevenko, G.N., Shangin-Berezovsky G.N. (1988). Skrytiy rezerv rosta i razvitiya zhivyh system. Vestnik sel’skokhozyaistvennoi nauki, 4(380), 96–105 (in Russian).

Akopyan, V.B., Stupin, A.Yu., Khabenskiy, B.M., Sergeeva, A.V., Mordvinova E.M. (2012). Sposob polucheniya mehanozavisimogo faktora rosta cheloveka. Patent RU 2523908 (in Russian).

Chisti, Y. (2003). Sonobioreactors: using ultrasound for enhanced microbial productivity. Trends in Biotechnology, 21(2), 89–93.

GOST R MEK 62127-1-2009. Obshchie trebovaniya k metodam izmerenii i sposobam opisaniya polei v chastotnom diapazone ot 0.5 do 40 MGz. (2010). Moscow: StandartInform (in Russian).

Lye, H.S., Khoo, B.Y., Karim, A.A., Rusul, G., Liong, M.T. (2012). Ultrasound enhanced growth and cholesterol removal of Lactobacillus fermentum FTDC 1311 in the parent cells but not the subsequent passages. Ultrasonics Sonochemistry, 19, 901–908.

Oleshkevich, A. (2015). Studies of frequency–dependent changes under modulated ultrasound exposure on cells in suspension. International Journal of Biomedicine, 4(1), 30–34.

Oleshkevich, A.A., Kutlikova, I.V., Cherenkova, I.A. (2015). Sravnitelniy analiz reaktsii raznyh vidov leikotsitov na obrabotku ultrazvukom minimalnoi terapevticheskoi intensivnosti. Proceed. Int. Conf. ‘Teoreticheskie i prikladnye voprosy nauki i obrazovaniya’. Tambov (in Russian).

Shigaeva, M.Kh. (2002). Raznoobrazie mikroorganizmov. Vestnik KazNU. Seriya biologicheskaya, 1, 133–137 (in Russian).

Tabatabaie, F., Mortazavi, S.A. (2010). Effects of ultrasound treatment on viability and autolysis of starter bacteria in hard cheese. American-Eurasian Journal of Agricultural & Environment Science, 8(3), 301–304.




DOI: http://dx.doi.org/10.15421/201659

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.




Since April 2018 Journal changed the editorial policy and starts to be published exclusively in English, and changed its main site into www.ujecology.com

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

© 2017 Ukrainian Journal of Ecology. ISSN 2520-2138