EFFECT OF NITROGEN NUTRITION SOURCES ON CAROTENOIDS SYNTHESIS FOR SOME BASIDIOMYCETES STRAINS

A. K. Veligodska, O. V. Fedotov, A. S. Petreeva

Abstract


The influence of certain nitrogen compounds - components of glucose-peptone medium (GPM) on the accumulation of carotenoids by some strains was investigated by surface cultivating basidiomycetes. The total carotenoid content was set in acetone extracts of mycological material spectrophotometrically and calculated using the Vetshteyn formula.

As the nitrogen-containing components used GPM with 9 compounds, such as peptone, DL-valine, L-asparagine, DL-serine, DL-tyrosine, L-proline, L-alanine, urea, NaNO3. The effect on the accumulation of specific compounds both in the mycelium and in the culture fluid of carotenoids by culturing certain strains of Basidiomycetes was identified.

Adding to standard glucose-peptone medium peptone at 5 g/l causes an increase of carotenoid accumulation by strain L. sulphureus Ls-08, and in a concentration of 4 g/l by strains of F. hepatica Fh-18 and F. fomentarius Ff-1201.

In order to increase the accumulation of carotenoids in the mycelium  we suggested to make a standard glucose-peptone medium with proline or valine for cultivating of L. sulphureus Ls- 08 strain; alanine for F. fomentarius Ff-1201 strain; proline, asparagine and serine - for strain Fh-18 of F. hepatica. The results can be implemented in further optimization of the composition of the nutrient medium for culturing strains of Basidiomycetes wich producing carotenoids.

Keywords: nitrogen-containing substances, Basidiomycetes, mycelium, culture filtrate, carotenoids


Full Text:

PDF

References


Пищевая биотехнология биологических продуктов из сырья растительного происхождения / О.А. Неверова, Г.А. Гореликова, В.М. Поздняковский // Новосибирск: Сиб. Универ. Из-ство, 2007 – 415 с.

Пирог Т.П. Загальна мікробіологія / Т.П. Пирог. – К.: НУХТ, 2010. – 623 с.

Физиологически активные соединения плодовых тел ксилотрофных базидиомицетов / В.Г. Бабицкая, В.В. Трухоновец, Д.А. Смирнов, В.В. Щерба, О.В. Осадчая, Т.В. Филимонова, Т.В. Черноок // Современная микология в России. Тезисы докладов второго съезда микологов России. – М., 2008. – С. 118-119.

Asatiani M.D. Higher basidiomycetes mushrooms as a source of antioxidants / M.D. Asatiani, G. Elisashvili, A.Z. Songulashvili, V. Reznick, S.P. Wasser // Progress in Mycology. – 2010. – P. 311–327

Wasser S.P. Medicinal mushroom Science: History, Current Status, Future Trends, and Unsolved problems / S.P. Wasser // Int. J. Med. Mush. – 2010. – 12 (1). – Р. 1–16.

Гесслер Н.Н. Участие ß-каротина в антиоксидантной защите грибной клетки. / H.H.Гесслер, A.B. Соколов, Т.А. Белозерская //Прикладная биохим. и микробиол. 2003. Т 39. № 4. с.427-429.

Ribeiro B. Do Bioactive Carotenoids Contribute to the Color of Edible Mushrooms? / B. Ribeiro, P. Guedes de Pinho, P. B. Andrade, C. Oliveira, A. César, S. Ferreira, P. Baptista, P. Valentão // The Open Chemical and Biomedical Methods Journal. – 2011. – №4 – P. 14-18.

Велигодська А.К. Порівняльна характеристика загального вмісту каротиноїдів у деяких видів базидіальних грибів / А.К. Велигодська, О.В. Федотов // Мікробіологія і біотехнологія. – Одеса: ОНУ ім.. І.І. Мечнікова, 2012. – №. 4(20). – С. 84-101.

Fedotov O.V., Veligodska A.K. Search producers of polyphenols and some pigments among Basidiomycetes // Biotechnologia Acta. – V. 7, No 1, 2014. – Р. 110-116.

Беккер 3.Э. Физиология и биохимия грибов. – М.: Изд-во Моск. ун-та, 1988. – 230 с.

Сааков В.С. Альтернативные пути биосинтеза каротиноидов у Procaryota и Eucaryota // Докл. АН России. – 2003. – Т. 392. – № 6. – С. 825–831.

Влияние различных источников азота и углерода на рост высших дереворазрушающих базидиальных грибов / В.М. Линовицкая, Л.П. Дзыгун, И.Р. Клечак, А.С. Бухало // Современная микология в России. Тезисы докладов второго съезда микологов России. – М., 2008. – С. 335.

Государственная Фармакопея СССР. – ХІ изд. – Вып. 1. – М.: Медицина, 1987. – 336 с.

Дудка И.А. Методы экспериментальной микологии. Справочник. / И.А. Дудка, С.П. Вассер, И.А. Элланская. – К.: Наук. думка, 1982. – 550 с.

Мусиенко М.М. Спектрофотометрические методы в практике физиологии, биохимии и экологии растений / М.М. Мусиенко, Т.В. Паршикова, П.С. Славный. – К.: Фитосоциоцентр, 2001. – 200 с.

Приседський Ю.Г. Статистична обробка результатів біологічних експериментів / Ю.Г. Приседський. – Донецьк: Кассиопея, 1999. – 210 с.

Федотов О.В., Волошко Т.Є. Розробка способів отримання і аналіз ферментних препаратів пероксидаз та каталаз деяких видів базидіоміцетів. // Біологічний вісник МДПУ ім. Богдана Хмельницького, 2013. № 1 (7), – С. 113-127.

REFERENCES

Neverova, O.A., Gorelikova, G.A., Pozdnyakovsky, V.M. (2007). Food biotechnology biological products from raw materials of plant origin. Novosibirsk: Siberian University.

Pirog, T.P. (2010). General microbiology. Kiev, NUHT.

Babickaya, V.G. Truhonovets, V.V., Smirnov, D.A., Szczerba, V.V. (2008). Physiologically active compounds of the fruiting bodies of xylotrophic basidiomycetes Modern Mycology in Russia. Abstracts of the Second Congress of the Russian mycologists.

Asatiani, M.D., Elisashvili, G., Songulashvili, A.Z., Reznick, V., Wasser, S.P. (2010). Higher basidiomycetes mushrooms as a source of antioxidants. Progress in Mycology. 4, 311–327

Wasser, S.P. (2010). Medicinal mushroom Science: History, Current Status, Future Trends, and Unsolved problems. Int. J. Med. Mush. 12 (1), 1–16.

Gessler, N.N., Sokolov, A.B., Belozerskaya, T. (2003). The participation of ß-carotene in antioxidant protection of fungal cells. Applied Biochemistry. and microbiology. 4 (39), 427-429.

Ribeiro, B., Guedes de Pinho, P., Andrade, P.B., Oliveira, C., César, A., Ferreira, S., Baptista, P., Valentão, P. (2011). Do Bioactive Carotenoids Contribute to the Color of Edible Mushrooms? The Open Chemical and Biomedical Methods Journal. 4, 14-18.

Velygodska, A.K., Fedotov, O.V. (2012). The comparative characteristic of general carotenoid content in some species of basidiomycetes. Microbiology and Biotechnology. 4 (20), 84-101.

Fedotov, O.V., Veligodska, A.K. (2014). Search producers of polyphenols and some pigments among Basidiomycetes. Biotechnologia Acta. 7 (1), 110-116.

Becker, Z.E. (1988). Physiology and biochemistry of fungi. Moscow: Moscow University Press.

Saakov, V.S. (2003). Alternative ways of carotenoid biosynthesis in Procaryota and Eucaryota. Reports Russian Academy of Sciences. (6) 392, 825-831.

Linovizkaya,V.M., Dzygun, L.P., Klechak, I.R. (2008) The effect of different sources of carbon and nitrogen on the growth of wood-destroying higher Basidiomycetes. Modern Mycology in Russia. Abstracts of the Second Congress of the Russian mycologists.

State Pharmacopoeia of the USSR. (1987). Moscow: Medicine.

Dudka, I.A., Wasser, S.P., (1982) Methods of Experimental Mycology. Kiev, Nauka.

Musienko, M.M., Parshikova, T.V., Slavniy, P.S. (2001) Spectrophotometric methods in the practice of physiology, biochemistry and ecology of plants. Kiev, Naukova Dumka.

Prisedsky, J.G. (2005). The software package for statistical analysis of the results ofbiological experiments. Donetsk: Donetsk National University.

Fedotov, O.V. Voloshko, T.E. (2013). Development of methods of obtaining and analyzing enzyme preparations peroxidase and catalase some species of Basidiomycetes. Bіologіcal Bulletin of Bogdan Chmelnytsky Melitopol State Pedagogical University. 3 (1), 113-127.




DOI: http://dx.doi.org/10.15421/20144_02

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.




Since April 2018 Journal changed the editorial policy and starts to be published exclusively in English, and changed its main site into www.ujecology.com

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

© 2017 Ukrainian Journal of Ecology. ISSN 2520-2138