LEVEL OF LIPID PEROXIDATION PRODUCTS IN THE BLOOD OF RATS UNDER THE INFLUENCE OF OXIDATIVE STRESS AND UNDER THE ACTION OF LIPOSOMAL PREPARATION OF "BUTASELMEVIT"

T. V. Martyshuk, B. V. Gutyj, O. I. Vishchur

Abstract


The article presents the results of investigation of the impact of stress on oxidative intensity of lipid per oxidation. It was proved that intramuscular injection of 50% solution of tetrachloromethane at a dose of 0.25 mL per 100 g of rat body causes the activation of free radical lipid oxidation with excessive accumulation of intermediate and final products of lipid peroxidation. Our results indicate that the development of oxidative stress leads to the significant acceleration of the formation and accumulation of lipid hydroperoxides and malondialdehyde (MDA) in plasma of rats. We registered the highest level of lipid hydroperoxides in rat blood plasma under oxidative stress on the second day of the experiment; it was 0.843 un/mL, whereas this index was 0.245 un/mL in the control group. We also revealed that the content of malondialdehyde was the highest in the experimental group on the fifth day of the experiment; it was almost 2 times higher than in control group.

We could recommend to apply the liposomal drug "Butaselmevit" which contains butafosfan, selenium, methionine, milk thistle, and vitamins for the inhibition of lipid peroxidation under the development of oxidative stress. It was proved that the parenteral injection of liposomal drug "Butaselmevit" to the rats for the development of oxidative stress leads to a reduction of peroxidation products level in their plasma. We revealed that the level of intermediate and final products of lipid peroxidation in the blood of rats that were used liposomal drug reached normal physiological values on the 14 day of the experiment. Our results suggested that the new liposomal drug "Butaselmevit" has definite antioxidant properties


Keywords


oxidative stress; rats; tetrachloride; lipid hydroperoxide; malondialdehyde; active forms of oxygen; liposomal drug "Butaselmevit"

Full Text:

PDF

References


Абрагамович О. О. Процеси ліпідної пероксидації при хронічних ураженнях печінки / О. О. Абрагамович, О. І. Грабовська, О. І. Терлецька // Медична хімія. - 2000. - Т. 2, № 1. - С. 5 8.

Антоняк Г. Л. Утворення активних форм кисню та система антиоксидантного захисту в організмі тварин / Г. Л. Антоняк, Н. О. Бабич, Л. І. Сологуб // Біологія тварин. - 2000. - Т. 2, № 2. - С. 34-43.

Барабой В.А. Перекисное окисление липидов и стресс / В.А.Барабой, И.И.Брехман, В.Г. Головитин // СПб.: Наука, 1992. - 268 с.

Бєленічев І.Ф. Антиоксидантна система захисту організму (огляд) / І.Ф. Бєленічев, Є.Л. Левицький, С.І. Коваленко // Современные проблемы токсикологии. - 2002. - № 3. - С. 29-31.

Влізло В. В. Лабораторні методи досліджень у біології, тваринництві та ветеринарній медицині : довідник / В. В. Влізло, Р. С. Федорук, I.Б. Ратич та ін.; за ред. В.В. Влізла. - Львів : Сполом, 2012. - 764 с.

Гутий Б.В. Вплив хлориду кадмію на інтенсивність процесів перекисного окиснення ліпідів та стан системи антиоксидантного захисту організму щурів / Б.В. Гутий // Вісник Сумського національного аграрного університету. - Суми, 2012. Випуск 7(31). - С. 31-34;

Скрипник І. М. Гепатопротекторні засоби в сучасній гепатології // Consilium Medicum Ukraina. - 2007. - №1(5). - С. 11-15.

Яценко О. Ю. Вплив рибіфлану на функціональний стан печінки шурів при хронічному ураженні тетрахлорметаном / О.Ю. Яценко, О.Л. Малоштан // Вісник фармації. - 2004. - №1 (37). - С. 67-70.

Batakov E. A. Effect of Silibum marianum oil and legalon on lipid peroxidation and liver antioxidant systems rats intoxicates with carbon tetrachloride. Eksp. Klin. Farmakol. - 2001. - 64. - P. 53-55.

Calabrese E., Leonard D. Zhao Xiaoqiang. Role of tissue repair in carbon tetrachloride hepatotoxicity in male and female Sprague-Dawley and Wistar rats. International Journal of Toxicology. 1999. - 15. - P. 62-69.

Cherkashina D. V., Petrenko A. Yu. Hepatoprotective effect of fetal tissue cytosol and its thermostable fraction in rats with carbon tetrachloride-induced hepatitis. Bulletin of Experimental Biology and Medicine. 2006. - 141 (4). - P. 544-547.

Lee J.Y. The preventive inhibition of chondroitin sulfate against the CCI4-induced oxidative stress of subcellular level / J.Y Lee, J.H. Lee, H.J. Kim [et al.) //Arch.Pharm.Res. - 2004. - Vol. 27, №3. - P. 340-345.

Li D. Effects of indoleamine 2,3-dioxygenases in carbon tetrachloride-induced hepatitis model of rats / D. Li, H. Cai, M. Hou, D. Fu [et al.] //Cell Biochem Funct. - 2012. - 30(4). - P. 309-314.

Official Journal of the European Union L276/33, 2010. DIRECTIVE 2010/63/EU OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 22 September 2010 on the protection of animals used for scientific purposes. 86/609/EC. 20.10.2010.

Saba A.B., Oyagbemi A.A., Azeez O.I. Amelioration of carbon tetrachloride-induced hepatotoxicity and haemotoxicity by aqueous leaf extract of Cnidoscolus aconitifolius in rats. Nig. J. Physiol. Sci. – 2010. - 25. - P. 139-147

Sato S., Dai W., Liu X.-L., Asano G. The protective effect of hepatocyte growth-promoting factor (pHGF) against carbon tetrachloride-induced acute liver injury in rats: an ultrastructural study. Medical Electron Microscopy. - 1999. - 32 (3). - P. 184-192

Weber L. Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model / L. Weber, M. Boll, A. Stampfl // Сrit. Rev. Toxicol. - 2003. - V.3, №2. - P. 105-136.

REFERENCES

Abragamovich, O.O., Grabovska, O.I., Terletska, O.I. (2000). Protsesi lipidnoyi peroksidatsiyi pri hronichnih urazhennyah pechinki. Medichna himiya, 2(1), 5–8. (in Ukrainian).

Antonyak, G.L., Babich, N.O., Sologub, L.I. (2000). Utvorennya aktivnih form kisnyu ta sistema antioksidantnogo zahistu v organizmi tvarin. Biologiya tvarin, 2(2), 34–43. (in Ukrainian).

Baraboy, V.A., Brehman, I.I., Golovitin, V.G. (1992). Perekisnoe okislenie lipidov i stress. Saint Petersburg: Nauka (in Russian).

Batakov, E. A. (2001) Effect of Silibum marianum oil and legalon on lipid peroxidation and liver antioxidant systems rats intoxicates with carbon tetrachloride. Eksp. Klin. Farmakol., 64, 53–55.

Belenichev, I.F. Levitskiy, E.L., Kovalenko, S.I. (2002) Antioksidantna sistema zahistu organizmu (oglyad). Sovremennye problemy toksikologii, 3, 29–31. (in Ukrainian)

Calabrese, E., Leonard, D. Zhao Xiaoqiang (1999) Role of tissue repair in carbon tetrachloride hepatotoxicity in male and female Sprague–Dawley and Wistar rats. International Journal of Toxicology, 15, 62–69.

Cherkashina, D. V., Petrenko, A. Yu. (2006) Hepatoprotective effect of fetal tissue cytosol and its thermostable fraction in rats with carbon tetrachloride–induced hepatitis. Bulletin of Experimental Biology and Medicine, 141(4), 544–547.

Gutiy, B.V. (2012). Vpliv hloridu kadmiyu na intensivnist protsesiv perekisnogo okisnennya lipidiv ta stan sistemi antioksidantnogo zahistu organizmu shchuriv. Visnik Sumskogo natsionalnogo agrarnogo universitetu, 7(31), 31–34 (in Ukrainian).

Lee, J. Y., Lee, J. H., Kim, H. J. (2004). The preventive inhibition of chondroitin sulfate against the CCI4–induced oxidative stress of subcellular level. Arch. Pharm. Res., 27(3), 340–345.

Li, D., Cai, H., Hou, M., Fu, D. (2012) Effects of indoleamine 2,3–dioxygenases in carbon tetrachloride–induced hepatitis model of rats. Cell Biochem Funct., 30(4), 309–314.

Official Journal of the European Union L276/33, 2010. DIRECTIVE 2010/63/EU OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 22 September 2010 on the protection of animals used for scientific purposes. 86/609/EC. 20.10.2010.

Saba, A. B., Oyagbemi, A. A., Azeez, O. I. (2010). Amelioration of carbon tetrachloride–induced hepatotoxicity and haemotoxicity by aqueous leaf extract of Cnidoscolus aconitifolius in rats. Nig. J. Physiol. Sci., 25, 139–147

Sato, S., Dai, W., Liu, X.–L., Asano, G. (1999) The protective effect of hepatocyte growth–promoting factor (pHGF) against carbon tetrachloride–induced acute liver injury in rats: an ultrastructural study. Medical Electron Microscopy, 32(3), 184–192

Skrypnyk, I. M. (2007) Gepatoprotektorni zasoby v suchasnij gepatologiyi. Consilium Medicum Ukraina, 1(5), 11–15. (in Ukrainian).

Vlizlo, V.V. (2012) Laboratorni metody doslidzhen u biologiyi, tvarynnycztvi ta veterynarnij medycyni. Lviv: Spolom (in Ukrainian).

Weber, L., Boll, M., Stampfl, A. (2003) Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachloride as a toxicological model. Сrit. Rev. Toxicol., 3(2), 105–136.

Yatsenko, O. Iu., Maloshtan, O. L. (2004) Vplyv rybiflanu na funktsionalnyi stan pechinky shuriv pry khronichnomu urazhenni tetrakhlormetanom. Visnyk farmatsii, 1(37), 67–70. (in Ukrainian).




DOI: http://dx.doi.org/10.15421/201631

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.




 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

© 2017 Ukrainian Journal of Ecology. ISSN 2520-2138