ADVANTAGES OF APPLICATION OF ALGORIZOBACTERIAL CONSORTIA IN CULTIVATION OF LEGUME CROPS

S. V. Didovich, І. А. Maltseva

Abstract


Possibility of creation of effective algobacterial consortia with high nodulе ability of rhizobial cells is experimentally proven. Pre-sowing bakterization treatment of chickpea seeds provided the increase of the productivity of plants such polyfunctional cyanorhizobial complex by 43,4% in the conditions of the field experiment. It may serve as a base for biological technology of growing chickpea for obtaining of ecologically safe production.

We brought under cultivation 12 strains associated with a strain of cyanobacteria Nostoc linckia 144, identified by their morphological, cultural, physiological, and biochemical properties. We revealed that on nitrogen-free substrate some 62 and 77% of strains satellite cyanobacteria and algae itself positively affect tuberization of soybeans and chickpeas and increase the dry weight of aboveground plant up to 28,6-35,7%; under condition of Southern black soils the bacterization of strains of Microbacterium laevaniformans KAGK and Mesorhizobium siceri raised the ground dry weight of plants per 0.82 g / plant (21.1%) compared with mono-farmery. We created the effective algological bacterial consortium of high activity of Mesorhizobium siceri cells, whose number was 9.7 h105 BUOD / mL, which exceed by two orders of magnitude higher the monoculture rhizobia. We proved experimentally the efficiency of usage of cyanobacteria combined with nodule bacteria in agrobiotechnology and suggested the high efficiency of prebacteryzation of seed by cyan-rhyzo-consortium of chickpeas which ensuring the increase of plant productivity by 43.4%.

Key words: chyanobacteria, nodule bacteria, consortia, legume crops, efficiency, productivity


Full Text:

PDF

References


Биопрепараты в сельском хозяйстве (Методология и практика применения микроорганизмов в растениеводстве и кормопроизводстве) (2005). (Ред. И. А. Тихонович, Ю. В. Круглова). Москва.

Бушулян, О. В., & Січкар, В.І. (2009). Нут: генетика, селекція, насінництво, технологія вирощування. Одеса.

Возняковская, Ю. М., & Попова, Ж.П. (1995). Методические указания по идентификации неспоровых бактерий, доминирующих в ризосфере растений. Л.

Експериментальна ґрунтова мікробіологія. (2010). (Ред. В. В. Волкогон). Київ: Аграрна наука.

Волкогон, В. В., Заришняк, А. С., & Гриник, І.В. (2011). Методологія і практика використання мікробних препаратів у технологіях вирощування сільськогосподарських культур. Київ: Аграрна наука.

Методы исследований клубеньковых бактерий. (1981). Методические рекомендации для курсов повышения квалификации научных сотрудников по сельскохозяйственной микробиологии. (Ред. Л. М. Доросинский). Л.

Определитель бактерий Бержи в 2-х т. (1997). (Перевод Г. Заварзина, ред. Дж. Хоулт, Н. Криг). Том 1. Москва: Мир.

Определитель бактерий Бержи в 2-х т. (1997). (Перевод Г. Заварзина, ред. Дж. Хоулт, Н. Криг). Том 2. Москва: Мир

Толкачёв, Н. З. (1990). Метод определения количества клубеньковых бактерий сои в почв. Труды ВНИИСХМ, 37–43.

Bashan, Yoav. (1998). Inoculants of plant growth-promoting bacteria for use in agriculture. Biotechnology Advances, 16(4), 729-770.

REFERENCES

Biopreparations in agricultures (Methodology and practice of the using microorganism in plant growing and feedproduction). (2005). (Editors I. A. Tihonovich, Yu. V. Kruglov). Moscow.

Bushulyan, O. V., & Sichkar, V. I. (2009). Chickpea: genetics, selection, seed-growing, technology of growing. Odesa.

Voznyakovskaya, Yu. M., & Popova, Zh. P.(1995). Methodical instructions on identification of nonsporen bacteria dominant in rhizosphere of plants. L.

Experimental ground microbiology. (2010). (Editor V. V. Volkogon). Kyiv: Agrarian science.

Volkogon, V. V., Zarishnyak, A. S., & Grynyk, I. V. (2011). Methodology and practice of the use of microbal preparations in technologies of growing of agricultural crops. Kyiv: Agrarian science.

Methods of researches of rhizobiums. Methodical recommendations for the extension course scientific employee on agricultural microbiology. (1981). (Editor L. M. Dorosinskiy). L.

Bergey's Manual of Determinative Bacteriology. (1997). (Trans .G. Zavarzin, editors John G Holt, N. Krieg). Vol. 1. Moscow: World.

Bergey's Manual of Determinative Bacteriology. (1997). (Trans .G. Zavarzin, editors John G Holt, N. Krieg). Vol. 2. Moscow: World.

Tolkachev, N. Z. (1990). Method of determining the quantity of soy-bean rhizobiums in soil. Proceedings of ARRIAM, 37–43.

Bashan, Y. (1998). Inoculants of plant growth-promoting bacteria for use in agriculture. Biotechnology Advances, 16(4), 729-770.




DOI: http://dx.doi.org/10.15421/20122_21

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM

Refbacks

  • There are currently no refbacks.




Since April 2018 Journal changed the editorial policy and starts to be published exclusively in English, and changed its main site into www.ujecology.com

 

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

© 2017 Ukrainian Journal of Ecology. ISSN 2520-2138