AN EFFECTIVE WAY TO CARRY OUT MASS IN VITRO PROPAGATION OF POTENTILLA ALBA L.

L. I. Tikhomirova, A. A. Kechaykin, A. I. Shmakov, O. V. Aleksandrova

Abstract


Despite the plant’s extensive area of distribution, Potentilla alba L. natural resources are scarce and cannot meet the modern needs of the pharmaceutical industry. Because of the mass preparation of medical raw materials by using P. alba, it entered into the list of rare and endangered species plants of the Red Data Book of the Republic of Belarus. This plant is not represented in the wild flora of Western Siberia, but there is a great need for developing a method for the mass propagation of P. alba using in vitro culture in order to obtain a high-quality planting material. At the explant stage, the technique of the P. alba introduction into in vitro culture is developed. This paper reveals the morphogenetic features of the development of P. alba explants of different types and the regenerative capacity of the tissue culture. At the micropropagation stage, the optimum culture media and the growth conditions for the regenerated plants are selected. At the stage of test-tube plants rooting and transferring them into ex vitro conditions, the most effective means of adaptation to non-sterile conditions in hydroponics are proposed.


Keywords


explant; regenerated plant; growth medium; micropropagation; rooting; adaptation.

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DOI: http://dx.doi.org/10.15421/201627

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