THE ENZYME ACTIVITY IN HEPATOPANCREAS OF CARP (CYPRINUS CARPIO) UNDER SULFONAMIDES’ INFLUENCE

Y. N. Kurbatova, V. M. Mykhalskaia, L. V. Malyuga, L. V. Gayova

Abstract


 

The results of studies of the effect of different concentrations of sulfonamides in the aquarium water on the activity of enzymes and protein metabolism in the hepatopancreas of carp yearlings were presented. It was found that the sulfanilamide added to the aquarium water at a concentration of 1.10; 3.15 and 6.30 mg / dm3 and 3 had no effect on the total protein content in the fish hepatopancreas during three days of exposure at first, second and third experimental groups compared with the control. It is proved that when the concentration of water in the aquarium sulfanilamide at a dose of 1.10 and 3.15 mg / dm3 in the hepatopancreas urea content compared with the control is not changed, while a dose of 6,30 mg / dm3 it had increased by 4%. Probably, such an increase in urea levels in the hepatopancreas of fish third test group associated with the activation of regulatory mechanisms in the body of carp to maintain a positive nitrogen balance and reduce the toxic effects of ammonia. We also did not reveal significant difference between the indices of glucose concentration in the hepatopancreas of carp experimental groups compared with the control. We founded that the activity of alanine aminotransferase and aspartate aminotransferase in hepatopancreas of carp experimental groups did not differ from controls, suggesting there was no effect of low concentrations of sulfonamides in the water after brief operation of the xenobiotic on the functional state of the hepatopancreas. Conducted experiments did not revealed changes in lipase activity as α-amylase and fish hepatopancreas in first, second and third experimental groups compared with the control. Their activity remained within the normal characteristic of the species and indicates that sulfanilamide virtually had no effect on the intensity of the hydrolytic processes in the intestines of fish. The performed investigations proved that carp yearlings were capable of adapting to minor concentrations of sulfonamides in water, as evidenced by its lack of effect on the total protein content, the concentration of glucose, urea and the activity of several enzymes in the hepatopancreas of fish.


Keywords


carp; sulfanilamide; total protein; urea; glucose; enzyme activity

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References


Авилов Ч. К. Ветеринарно-санитарная оценка отходов свиноводческого комплекса / Ч.К. Авилов, А.А. Денисов // Ветеринария. – 2000. – №12. – С. 49–52.

Биологическая химия / Е.С. Северина, Т.Л. Алейникова, Е.В. Осипов, С.А. Силаева. – М.: Медицинское информационное агентство, 2008. – 364 с.

Дорогов С.М. Фармакология / С. М. Дорогов. – Харьков: Издательский центр ХАИ. – 2002. – 280 с.

Іванова О.В., Захаренко М.О. Санітарно-гігієнічна оцінка стоків тваринницьких підприємств // Ветеринарна біотехнологія. – 2010. – № 17. – С. 82–87.

Камышников B.C. Справочник по клинико-биохимической лабораторной диагностике / Камышников B.C. – Минск: Беларусь, 2000. – 463 с.

Кокунин В.А. Статистическая обработка при малом числе опытов. / Кокунин В.А. // Укр. биохим. журн. - 1975. – т. 47. – № 6. – С. 776–790.

Курбатова І.М., Цедик В.В., Михальська В.М., Малюга Л.В. До питання про якість води водойм рибогосподарського призначення ті її вплив на розвиток ікри коропа (Cuprinus carpio L.// Наук. віс. Львів. нац. ун- ту вет. мед. і біотех. 2008. – №4 (39). – С. 273–278.

Курбатова І.М., Цедик В.В., Свириденко Н.П. Розвиток ікри та виживання ембріонів коропа за дії альбендазолу / Наук. віс. НУБіП України.– К., 2013. -193. – С. 127–131.

Мудра А.Є., Фальфушинська Г.І., Столяр О.Б. Інтегральний аналіз стану антистресорних систем гепатоцитів прісноводних тварин за дії пошкоджуючих чинників середовища // Наук. зап. Терноп. педун-ту. Сер. Біологія. – Тернопіль, 2008. – № 2 (36). -С. 151–160.

Мурадова Г.Р., Рабаданова А.И. Динамика содержания белков в сыворотке крови сеголеток карпа при хроническом воздействии тяжелых металлов // Успехи совр. естествознания. – 2012. – №7. -С. 58–62.

Цудзевич Б. О., Столяр О. Б., Калінін І. В., Юкало В. Г. Ксенобіотики: накопичення, детоксикація та виведення з живих організмів / – Тернопіль. Вид-во Терн. Нац. Тех. Ун-ту. 2012. – 384 с.

Akbaba Y., Akıncıoğlu A., Göçer H., Göksu S. Carbonic anhydrase inhibitory properties of novel sulfonamide derivatives of aminoindanes and aminotetralins // Journal of Enzyme Inhibition and Medicinal Chemistry. – 2014. – Vol. 29, - № 1. – P. 35-42.

Barreteau H., Simcic M., Sink R., Cesar J. Second-generation sulfonamide inhibitors of d-glutamic acid-adding enzyme: аctivity optimisation with conformationally rigid analogues of d-glutamic acid. // European Journal of Medicinal Chemistry. - 2011. -Vol. 46. - № 7. – P. 2880–2894.

Białk-Bielińska A., Stolte S., Arning J, Uebers U. Ecotoxicity evaluation of selected sulfonamides. // Chemosphere. – 2011. – Vol. 85. – № 6. – P. 928–933.

Ching-Hua Huang, David L. Sedlak. Analysis of estrogenic hormones in municipal wastewater effluent and surfaces water using enzyme-linked immunosorbent assey and gas chromatography tandem mass spectrometry. Environmental toxicology and chemistry, 2001 – Vol. 20. – № 1. – P. 133–139.

Gornelly S. Determination of serum protein by mean of the biuret reaction / Gornelly S. // J. Biol. Chem. – 1949. – Vol. 177. – № 2. – P. 751–755.

Gulkowska A., Leung H.W., So M.K., Taniyasu S., Yamashita N., Yeung L.W., Richardson B.J., Lei A.P., Giesy J.P., Lam P.K. Removal of antibiotics from wastewater by sewage treatment facilities in Hong Kong and

Shezhen, China // Water research, 2008, Vol. 42 (1-2). P. 395-403.

Göçer H., Akıncıoğlu A., Öztaşkın N., Göksu S., Gülçin İ. Synthesis, antioxidant, and antiacetylchoalinesiterase activities of sulfonamide derivatives of dopamine-related compounds. // Arch. Pharm. – 2013. – Vol. 346, № 11. – P. 783–792.

Patel D., JainM, Shah S., Bahe R. Discovery of potent, selective and orally bioavailable triaryl-sulfonamide based PTP1B inhibitors // Bioorganic & Medicinal Chemistry Letters. – 2012. – Vol. 22, - № 2, - P 1111–1117.

REFERENCES

Akbaba, Y., Akıncıoğlu, A., Göçer, H., Göksu, S. (2014). Carbonic anhydrase inhibitory properties of novel sulfonamide derivatives of aminoindanes and aminotetralins. Journal of Enzyme Inhibition and Medicinal Chemistry, 29(1), 35–42.

Avylov, Ch.K., Denisov, A.A. (2000). Animal health assessment pig farm waste. Veterinary science, 12, 49–52 (in Russian).

Barreteau H., Simcic M., Sink R., Cesar J. Second-generation sulfonamide inhibitors of d-glutamic acid-adding enzyme: аctivity optimisation with conformationally rigid analogues of d-glutamic acid. // European Journal of Medicinal Chemistry. - 2011. -Vol. 46. - № 7. – P. 2880–2894.

Białk-Bielińska, A., Stolte, S., Arning, J, Uebers, U. (2011). Ecotoxicity evaluation of selected sulfonamides. Chemosphere, 85(6), 928–933.

Dorohov, S.M. (2002). J. Pharmacology. Kharkov: Kharkov University Publishing Center (in Russian).

Göçer, H., Akıncıoğlu, A., Öztaşkın, N., Göksu, S., Gülçin, İ. (2013). Synthesis, antioxidant, and antiacetylchoalinesiterase activities of sulfonamide derivatives of dopamine-related compounds. Arch. Pharm, 346(11), 783–792.

Gornelly, S. (1949). Determination of serum protein by mean of the biuret reaction. J. Biol. Chem, 177(2), 751–755.

Gulkowska, A., Leung, H.W., So, M.K., Taniyasu, S., Yamashita, N., Yeung, L.W., Richardson, B.J., Lei, A.P., Giesy, J.P., Lam, P.K. (2008). Removal of antibiotics from wastewater by sewage treatment facilities in Hong Kong and Shezhen, China. Water research, 42(1–2), 395–403.

Huang, Ching-Hua, Sedlak, D.L. (2001). Analysis of estrogenic hormones in municipal wastewater effluent and surfaces water using enzyme-linked immunosorbent assey and gas chromatography/tandem mass spectrometry. Environmental toxicology and chemistry, 20(1), 133–139.

Ivanova, O.V, Zakharenko, M.O. (2010). Sanitary-hygienic evaluation of wastewater livestock enterprises. Veterinary Biotechnology, 17, 82 – 87 (in Ukrainian).

Kamyshnikov, B.C. (2000). Handbook of clinical and biochemical laboratory diagnostics. Minsk: Belarus (in Russian).

Kokunin, V.A. (1975). Statistical analysis of a small number of experiments. The Ukrainian Biochemical Journal, 47(6), 776–790 (in Russian).

Kurbatova, I.M., Tsedyk, V.V, Michalska, V.M., Malyuga, L.V. (2008). On the quality of water reservoirs industrial fishing are its effects on the development of carp roe (Cuprinus carpio L.). Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies, 4 (39), 273–278 (in Ukrainian).

Kurbatova, I.M., Tsedyk, V.V., Sviridenko, N.P. (2013). The development of eggs and embryos survive carp for the actions of albendazole. Scientific Journal of NULES of Ukraine, 193, 27–131 (in Ukrainian).

Mudra, A.E., Falfushynska, G.I., Carpenter, O.B. (2008). Integral analysis of hepatocytes antystresornyh freshwater animals for actions damaging environmental factors. Scientific Issue of Ternopil Volodymyr Hnatiuk National Pedagogical University. Series: Biology, 2(36), 151–160. (in Ukrainian).

Muradova, G.R., Rabadanova, A.I. (2012). Dynamics of proteins in the blood serum of carp fingerlings in chronic effects of heavy metals present-day successes. Advances in Current Natural Sciences, 7, 58 – 62 (in Russian).

Patel D., JainM, Shah, S., Bahe, R. (2012). Discovery of potent, selective and orally bioavailable triaryl-sulfonamide based PTP1B inhibitors. Bioorganic & Medicinal Chemistry Letters, 22(2), 1111–1117.

Severyna, E.S., Aleinykova, T.L., Osypov, E.V., Sylaeva, A.S. (2008). J. Biol. Chem. Moscow: Medical News Agency (in Russian).

Tsudzevych, B.A., Carpenter, A.B., Kalinin, I.V., Yukalo, V.G. (2012) Xenobiotics: accumulation, detoxification and excretion of organisms. Ternopil. Izdatel'stvo Ternopilskogo universitetu.




DOI: http://dx.doi.org/10.15421/2016107

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