EFFECT OF GLUTAMATE MANGANESE ON THE REPRODUCTIVE FUNCTION AND EMBRYOGENESIS OF FEMALE RATS

O. V. Shtapenko, I. I. Hevkan, Yu. I. Slyvchuk, V. I. Syrvatka, S. V. Fyodorova

Abstract


Pregnancy is associated with increased nutritional needs due to the physiologic changes of the female and the metabolic demands of the embryo/fetus. The use of chelating compounds with high biological activity increased the fertility of female rats by stimulation metabolism and functional activity of the reproductive system. Manganese is an essential element utilized by antioxidants, including superoxide dismutase (MnSOD), and others metalloenzymes that take part in reduction reactions, in multiple physiological processes including reproductive system. The aim of the research was to determine the effect of subcutaneous injections of Mn glutamate in liposomal forms on the reproductive system and the process of embryogenesis of experimental female rat.

The study was conducted on female rats aged 2.5-3 months with body weight of 180-200 g. Rats with dated gestation were divided into three groups: two experimental and control. Female rabbits of the 1th experimental group were subcutaneous injected of 2 mg/ml for Mn glutamate in liposomal form one week before fertilization and the animals of the 2th group were obtained the same preparation during fertilization. Rats were euthanized on the twentieth day, ovariens were singled out of fiber, visual inspection and counting the number of yellow bodies were performed, absolute and relative mass indexes were determined, Mn glutamate influence index was calculated.

Experimental results showed that the administration of Mn glutamate 7 days before fertilization and during fertilization significant increased the number of corpora lutea of pregnancy (p<0,001), number of live fetuses (p<0,001) due to the decrease in general and pre-implantation embryonic mortality compared with the control group. The experimental results showed improvement in key indicators of embryonic development. We observed significant increase in the number of implanted embryos on 1 female at 10,7±0,26 and 11,3±0,21 (p<0,001) (9,4±0,16 vs. control group) and decrease of the number of resorption in the female rats treated with Mn glutamate as compared with the control group. The results suggest a beneficial effect on the course of Mn glutamate embryogenesis of experimental animals.

Key words: glutamate manganese, pregnancy, embryogenesis, liposomal preparation


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References


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DOI: http://dx.doi.org/10.15421/2015024

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